本論文整合以甲苯為主要基質好氧共代謝三氯乙烯之現地及實驗室研究。在現地擠注甲苯蒸氣及空氣於含水層，成功馴養現地甲苯分解菌，有效共代謝降解三氯乙烯。擠注甲苯蒸氣成功解決甲苯液體注入井附近因微生物過度成長所造成的井篩阻塞問題。在實驗室使用與模場含水層相同之河川黑砂（未受三氯乙烯污染）作為供試土壤，應用半連續培養泥漿法（semicontinuous slurry microcosm method）以甲苯馴養現地模場微生物族群，以微觀的角度深入探討甲苯分解菌好氧共代謝三氯乙烯的生物程序，比較實驗室微生態系與現地模場三氯乙烯生物降解之成效。根據微生物16S rDNA分子生物技術鑑定生物處理系統中甲苯分解菌，分離菌株HYL-QT1及HYL-QT2分別與Ralstonia sp. P-10及Pseudomonas putida相似度達99%及100%。根據單一菌株批次試驗結果顯示HYL-QT1（Ralstonia sp. P-10）及HYL-QT2（Pseudomonas putida）共代謝三氯乙烯一階反應速率常數皆為0.5/day。實驗室微生態系試驗三氯乙烯生物共代謝效率為46%。現地甲苯分解菌三氯乙烯生物共代謝效率高達90%以上。現地及實驗室三氯乙烯生物共代謝效率差異主要關鍵為甲苯存在生物處理系統有效反應時間的不同所致。在實驗室微生態系試驗中，甲苯存在的有效反應時間僅有1天；在現地模場試驗中，甲苯存在的有效反應時間為3天。根據甲苯基質多次注入試驗結果證實甲苯存在生物處理系統的有效反應時間愈長，三氯乙烯生物共代謝降解效率愈高。

The aim of this study is to integrate laboratory and pilot studies of aerobic cometabolism of TCE using toluene as the primary substrate. Delivery of toluene in a vapor state with air enhanced the growth of indigenous toluene-utilizing bacteria that would degrade TCE by aerobic cometabolism. Meanwhile, delivering toluene in a vapor state effectively reduced potential clogging near the injection points due to excessive microbial growth, which was observed in the field when the injection of neat toluene was employed. Using the same clean river sand employed for aquifer material at the in-situ pilot study, a semicontinuous slurry microcosm method was applied to confirmed the process of aerobic cometabolism of TCE using toluene as the primary substrate. Based on the nucleotide sequence of 16S rRNA genes, the toluene-utilizing bacteria in microcosms were identified, i.e., Ralstonia sp. P-10 and Pseudomonas putida. The first-order constant of TCE-degradation rate was 0.5/day for both Ralstonia sp. P-10 and Pseudomonas putida. The TCE cometabolic-biodegradation efficiency measured from the slurry microcosms was 46%. And over 90% removal of TCE was observed from the in-situ pilot study. The difference in the TCE cometabolic-biodegradation efficiency was likely due to the reactor configurations and the effective time duration of toluene presence in laboratory microcosms（1days）vs. in-situ pilot study（3 days）. The results of microcosm experiments using different toluene-injection schedules supported the hypothesis. With a given amount of toluene injection, it is recommended to maximize the effective time duration of toluene presence in reactor design for TCE cometabolic degradation.

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