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研究生： 許筱芬
Hsu, Hsiao-Fen
論文名稱： 藉由空間侷限調控膠原蛋白與玻尿酸之層級式共組裝
Hierarchical Co-Assembly of Collagen with Hyaluronic Acid through Spatially-Confined Structures
指導教授： 李介仁
Li, Jie-Ren
學位類別： 碩士
Master
系所名稱： 理學院 - 化學系
Department of Chemistry
論文出版年： 2021
畢業學年度： 109
語文別： 中文
論文頁數： 99
中文關鍵詞： 細胞外間質共組裝 、空間侷限流體 、逐層沉積技術
外文關鍵詞： ECM co-assembly, spatially-confined, layer-by-layer
相關次數： 點閱：101  下載：0 
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    • 目錄 1 圖目錄 3 第一章 緒論 6 1.1 前言 6 1.2 細胞外間質(Extracellular Matrix, ECM) 6 1.2.1 第一型膠原蛋白 8 1.2.2 玻尿酸 10 1.3 蛋白質微奈米製程 11 第二章 探討第一型膠原蛋白與玻尿酸之共自組裝行為 16 2.1 前言 16 2.2 實驗部分 17 2.3 結果與討論 20 2.3.1 第一型膠原蛋白在雲母片上自組裝之型態 20 2.3.2 第一型膠原蛋白溶液培育時間對其在雲母片上自組裝的影響 20 2.3.3 玻尿酸在雲母片上自組裝之型態 21 2.3.4 第一型膠原蛋白與玻尿酸在雲母片共組裝之型態 21 2.3.5 第一型膠原蛋白與玻尿酸溶液培育時間對其在雲母片上共組裝的影響 22 2.3.6 玻尿酸濃度對第一型膠原蛋白與玻尿酸在雲母片上共組裝的影響 23 2.3.7 玻尿酸分子量對第一型膠原蛋白與玻尿酸在雲母片上共組裝的影響 25 2.3.8 離子強度對第一型膠原蛋白與玻尿酸共組裝的影響 29 2.3.9 培養溫度對第一型膠原蛋白與玻尿酸共組裝之影響 30 第三章 探討第一型膠原蛋白與玻尿酸在膠原蛋白奈米纖維結構上之共組裝行為 32 3.1 前言 32 3.2 實驗部分 33 3.3 結果與討論 37 3.3.1 玻尿酸分子量對第一型膠原蛋白與其在奈米纖維結構上共組裝的影響 37 3.3.2 固定劑對第一型膠原蛋白與玻尿酸在奈米纖維結構上共組裝的影響 38 3.3.3 玻尿酸分子量對第一型膠原蛋白與其在固定後奈米纖維結構上共組裝的影響 39 第四章 利用PDMS模板控制空間侷限流體內第一型膠原蛋白與玻尿酸共組裝形成多層結構 41 4.1 前言 41 4.2 實驗部分 42 4.3 結果與討論 47 4.3.1 不同空間侷限對第一型膠原蛋白與玻尿酸共組裝的影響 47 4.3.2 玻尿酸分子量對PDMS模板控制膠原蛋白與其共組裝的影響 49 4.3.3 玻尿酸分子量對奈米纖維結構引導膠原蛋白與其在空間侷限流體內共組裝的影響 51 4.3.4 固定劑對奈米纖維結構引導膠原蛋白與玻尿酸在空間侷限流體內共組裝的影響 53 4.3.5 空間侷限奈米纖維結構引導膠原蛋白與玻尿酸在空間侷限流體內共組裝 53 第五章 利用不同幾何結構的PDMS模板控制空間侷限流體內第一型膠原蛋白自組裝形成多層結構 56 5.1 前言 56 5.2 實驗部分 57 5.3 結果與討論 60 5.3.1 探討空間侷限流體內的第一型膠原蛋白奈米纖維於不同幾何結構上自組裝 60 5.3.2 探討不同的空間侷限流體內第一型膠原蛋白奈米纖維於相同幾何結構上自組裝 61 第六章 結論 64 第七章 未來方向：異種第一型膠原蛋白自組裝行為 67 7.1 實驗部分 67 7.2 結果與討論 69 7.2.1 探討(魚)第一型膠原蛋白在雲母片上自組裝之行為 69 參考文獻 71 圖附錄 75

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