本研究著重於評估不同麴菌的麴酸生產能力，從中選擇生產能力較佳者來探討醱酵條件對菌體生長型態與麴酸生產能力，並進一步討論在醱酵槽加裝不織布對麴酸生產的影嚮。所採用的不織布為”Polyfilling”噴交棉 (樹脂棉)，乃利用PET回收料，經過再加工生產的聚酯再生棉。
在本研究所測試之5株黴菌菌株中，只有A. flavus CCRC 30010及A. oryzae CCRC 30102有較顯著量之麴酸產生，分別為24.4及6.86 g/l。而在YES broth中振盪培養時A. flavus CCRC 30010的菌體生長雖不如A. oryzae CCRC 30102，但其麴酸產量卻為A. oryzae CCRC 30102的5倍左右。當起始pH為6.5時A. flavus CCRC 30010麴酸之產生最佳而菌體之生長以pH為4.5時最佳。培養溫度則以25℃培養時，麴酸之產生與菌體之生長最佳。當以sucrose 為碳源時，A. flavus CCRC 30010麴酸之產量及菌體之生長最佳。氮源中以yeast extract 對麴酸之產生與菌體之生長最佳。在YES broth中，KH2PO4、 K2HPO4、 (NH)4HPO4、MgSO4˙7H2O之添加對A. flavus CCRC 30010麴酸及菌體之產生並無顯著性之影嚮。
在吾人之懸浮細胞批次醱酵槽實驗中，菌體於第3天才進入對數生長期末期，此時麴酸亦開始產生。而在接近第10天時麴酸的產量可達最高，其值為8.g/l。然而，在不織布系統中，麴酸生產的時間雖較晚（第4天）但在第11天時麴酸產量竟達20.85g/l。吾人亦得到一個結果，不織布的存在下可以增加菌絲體的完整性，有利麴酸的產生，使得菌體在醱酵槽中，以類似靜置培養的方式，能更快、更有效的生產麴酸。

This study intended to evaluate the productivity of different strains of fungi and to select a kojic acid producing strain with high productivity can be studied. Then we intend to produce kojic acid in the presence of nonwoven fabric and to investigate the effect. We used a nonwoven fabric (with the trade name of Polyfiling), which consists of PET.
Aspergillus flavus CCRC 30010 and A. oryzae CCRC 30102 produced significant highest amount of kojic acid (24.4&6.86 g/l) among the five tested strains of mold. More kojic acid production by A.flavus 30010 was noted in YES broth in shaking culture than A. oryzae CCRC 30102, although growth is less. YES broth with an initial pH of 6.5or a cultivation temperature of 25℃would result in the higher production of kojic acid by A. flavus CCRC 30010. While growth of A. flavus CCRC 30010 was best when cultivation was done with YES having an initial of pH of 4.5 and at 25℃. Greater growth and kojic acid production were observed when sucrose was used as the carbon source. Yeast extract was the best nitrogen source for the growth and kojic acid production by A. flavus CCRC 30010. In addition, it was found that addition of KH2PO4、 K2HPO4、 (NH)4HPO4、MgSO4˙7H2O to YES broth had no significant effects.
In the suspension fermentation, the cells entered the exponential growth end phase at 3 day, and the kojic acid production. The maximum kojic acid was 8g/l at 11 day. In contrast, the kojic acid was 20.85 at 11 day if using the non-woven-fabrics system. Based on these findings, the roles of the non-woven fabrics in the fermentation with A. flavus CCRC 30010 an improve completely of mold in the fermentation, and production kojic acid like static culture rapidly and effectively.

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