磷脂醯絲胺酸受體 (Phosphatidylserine Receptor, PSR) 具有辨識凋亡小體 (apoptotic body) 的功能與吞噬的能力，但是其如何動態表現再斑馬魚胚胎內尚未清楚。因此本論文將選殖 PSR 啟動子並與報導基因 (EGFP) 接合，以便在斑馬魚胚胎發育時直接動態觀察 PSR 在不同時間與空間內之表現。首先，我們證實斑馬魚 PSR 上游 4 kbps 序列具有啟動子區域，可以在斑馬魚胚胎中啟動綠螢光基因。在 PSRP-4K-EGFP 基因轉殖魚發現，綠色螢光基因在卵子時期到孵出時期皆有表現，主要在體節、眼、腦部、心臟、孵化腺與泌尿道開口，皆有明顯的綠色螢光表現。利用此綠色螢光報導系統，我們首次發現於受精後 14 天後的幼魚骨骼及在成魚的精巢和卵巢皆有綠色螢光蛋白的表現。接著以連續刪除方式將 PSR promoter 切割成不同大小的片段，使用 EGFP 與 Luciferase 兩種報導基因的方式，發現在 psr 基因 start cordon 上游 300 bps 是啟動基因最重要的功能區域，並且推測 repeat 片段 (約 3 kb) 可能具有 enhancer 的功能。本篇的研究發現 PSR 不只對於胚胎發育時期重要，而且在後期骨骼與生殖腺的發育有重要關係。

The general function of phosphatidylserine receptor that PSR can recognize the apoptotic bodies for clearance of corpse cells, but they temporally and spatially expressive pattern is still unclear. In this thesis, we cloned and constructed the PSR promoter that fused with green fluoresce protein for directly monitoring the temporal and spatial profile of PSR expression during zebrafish embryogenesis. We demonstrated that the 4kb of upstream sequence of PSR gene, which contain promoter region that can drive report gene EGFP expression in early zebrafish embryos. In the PSRP-4K-EGFP transgenic zebrafish fish line (F1), EGFP was expressed from oocyte to hatch out stages, especially, apparently expressed in somites, brain, eyes, heart, hatching gland and open of urinary duct. Interestingly, we first found that the PSR gene can express in skeleton (at the 14 dpf larvae) and testis or ovary (at adult stages). Furthermore, we constructed the different fragments of PSR promoter for the promoter activity assay. In the luciferase reporter assay, we found that a minimal requirement for PSR promoter activity is located in upstream 300 bps of start codon, and in the 3 kbps of upstream sequence that contain repetitive sequence may exist the enhancer elements. In this study, we discovered the PSR is not only important in embryogenesis, but also involved in skeletogenesis and gonadogenesis in late development stages.

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