養殖漁業的價值，正因傳統捕撈漁業日漸飽和的影響，越來越受到重視，而魚隻的價格則受到肉質與賣相的影響。先前的研究發現，sparc (secreted protein acidic and rich in cystein /osteonectin )基因所轉譯出的SPARC 蛋白主要分布在骨
頭與細胞間質中，具有調節細胞與細胞外基質黏附性及細胞訊息調控的功能，其所掌控的生理現象早至胚胎發育過程，細胞的分化、組織的鈣化生成、骨骼發育、型態及器官發育有極大影響，推測很有可能與早期魚類發育過程中骨骼的生長有關係，會影響到魚隻的賣相。在哺乳類的研究之中，也發現SPARC 基因具有調節脂肪形成的能力，不過結果卻很兩極化。在本研究計畫中，將就SPARC 蛋白對於魚類細胞的功能與作用機制進行探討，結果顯示，SPARC 具備與鈣離子結合的能力，並且鈣離子對其功能具有很高的重要性；並觀察在胚胎發育時期、病毒感染壓力下與魚體內各組織的基因表現量，以了解SPARC 在生理功能上所扮演的角色，由結果可知，SPARC 的表現大多出現在胚胎發育初期，在較大的魚隻中，則多出現在鰓、眼睛、小腸等與外界接觸的組織和肌肉，顯示期生理上功能或許對於組織的重塑扮演重要的角色。

Because of the saturation of traditional fishing, aquaculture is becoming more and more important. The value of cultured fish is decided by its appearance and taste.
Previously study has found that SPARC (secreted protein acidic and rich in cystein /osteonectin) protein, translated by sparc gene, is mostly expressed in bone and ECM, could modulate the interaction between cells and extracellular matrix and regulate signal transduction. SPARC is involved in the development of ossified and
mineralized tissues, tissue morphogenesis and cellular differentiation. Probably SPARC can affect the appearance of marketing fish. Besides, previous studies also showed that SPARC may regulate fat production, but the results can be polarized into two directions. In this project, we studied the functions and mechanisms of SPARC protein in fish cell and also discussed the physiological importance by observing the expression level during embryo development, virus infection and expression levels in
various tissues. The results suggest that SPARC protein can binds to calcium ion and the binding of calcium ion is vital to SPARC function. Even the recombinant protein
of SPARC EC (extracellular calcium binding) domain can show a minor anti-spreading activity to GF-1 cell. As a multifunctional ECM (extracellular matrix) modulator, SPARC expressed more on the early stage of embryo development and
mainly in tissues contact with external environment, thereby influences many important physiological and pathological processes.

廖中健，點帶石斑魚sparc 基因選殖及特性分析，國立成功大學生物
科技研究所碩士論文，2006

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