N-甲基吡咯烷酮（NMP）為一種在半導體產業被廣泛應用的有機溶劑。NMP能夠使用好氧或是厭氧生物處理方式進行處理，而一般的研究著重於好氧處理。厭氧生物處理NMP相對來說是一種較為新穎的方式。因此，本研究的目的是為了評估NMP在厭氧情況下的降解可行性。此外，由於較少相關資料，NMP在厭氧情況下的降解途徑以及厭氧情況下的NMP降解菌群研究也是本研究的目的之一。在最高為375 mg/L-day的有機負荷下，CSTR的操作可以達到90%以上的COD去除效率。然而在有機負荷提升至400 mg/L-day以上時會導致處理效率下降。AFMBR能夠在有機負荷為325 mg/L-day 且NMP的負荷在100 mg/L-day的情況下達到平均95%以上的COD去除效率。經過批次實驗的測試後證實了高濃度的NMP會對其降解造成抑制，而其抑制模式亦符合Haldane equation。在一個進行甲烷菌抑制的批次實驗顯示甲烷菌在NMP的厭氧降解途徑中扮演重要的角色，抑制甲烷菌所導致甲基還原酶的基質累積或許會對NMP降解菌群形成抑制作用。從次世代定序的結果來看抑制甲烷菌亦會使其微生物族群產生變化。在降解途徑的分析中，抑制甲烷菌會導致乙酸的累積，並顯示此污泥中至少含有3種不同的微生物族群。

N-methylpyrrolidone is a common organic solvent in the semiconductor industry wastewater, which can be treated by either aerobic or anaerobic biological processes. Compare to the typical way, which is the aerobic biological treatment, the anaerobic treatment is a novel process on the NMP biodegradation. Therefore, the purpose of this study is to evaluate and improve the removal efficiency of NMP in CSTR and AFMBR under anaerobic conditions. The metabolic pathway of NMP and the microbial community of the NMP degrading microorganism were also the aims of this study. The CSTR can reach an average of 90% removal efficiency in its operation period with a 375 mg/L-day as maximum OLR. An inhibition phenomenon happened when the OLR is above 400 mg/L-day. The AFMBR can get an average of 95% removal efficiency with a 325 mg/L-day OLR and 100 mg/L-day NMP loading rate. A series of batch experiments proved that a high concentration of NMP would inhibit its degradation. The inhibition batch experiment showed that methanogens might play an important role in the NMP metabolic pathway. The accumulation of methylreductase’s substrate may inhibit the NMP degrading microorganism. Various of volatile fatty acids were detected as a metabolite.

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