電漿是具有生物材料應用潛力的第四態,其可以選擇性地促使癌細胞凋亡,並
且同時不影響正常細胞的生長。大量研究顯示各種癌細胞在暴露於非熱微電漿後經 歷凋亡之現象,這是由於在電漿處理期間所產生的活性氧和活性氮物質和細胞作用 所造成。然而,鮮少關於在不同工作氣體下的癌細胞處理之研究。因此,在本研究 中,會比較氦氣與氦氣/氮氣混合電漿對三種頭頸癌細胞,即 SAS,CAL 27 和 FaDu 之抗癌效果。此外,亦整理其他使用不同工作氣體但相似電漿機台的研究數據與本 研究做比較,進而瞭解對於誘發細胞凋亡較佳的工作氣體組合。
結果表明,非熱微電漿確實誘導了由活性物質產生引起的頭頸癌細胞凋亡。於 工作氣體中添加氧氣或氮氣,可以在細胞溶液中和細胞本體內產生更多的活性氧或 活性氮物質,進而促使更高的癌細胞凋亡率。此外,空氣電漿比氮氣電漿誘導更高 的癌細胞凋亡比例。由於潘寧效應,氦氣比氬氣有更高的電解離效果。又在非熱的 電漿暴露下,癌細胞凋亡主要由活性氧引起。因此,為了提高癌細胞的處理效果, 應將水或氧氣加入氦氣中。

Plasma is the fourth state of matter with tremendous biomaterial application potential, selectively targeting cancer cells while leaving normal cells intact. A great number of studies showed positive results of various cancer cells undergoing apoptosis after being exposed to plasma due to the actions of reactive oxygen and nitrogen species (ROS/RNS) generated during plasma treatment. However, there is a lack of comparison on the treatment effectiveness of plasma with different feed gases. In this study, non-thermal plasmas (NTP) generated under different feed gases were employed to treat three head and neck cancer cell lines, namely SAS, CAL 27 and FaDu, and a comparison of the anticancer effect was made. Moreover, studies using similar plasma jets but with different operating parameters and working gases were also reviewed and compared.
The results show that NTP does induce head and neck cancer cells apoptosis caused by the reactive species generated. More ROS/RNS were generated in the medium and within the cells with the addition of oxygen/nitrogen in the plasma working gas stream. In addition, air plasma induces higher cancer cell apoptosis than N2 plasma. He plasma shows better ionization than Ar plasma due to Penning effect. Under the exposure of NTP, cancer cell apoptosis is caused mainly by ROS. Thus, to improve the treatment effectiveness, H2O and O2 should be added to the He feed gases.

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