本研究利用陰離子與陽離子界面活性劑經沉澱法得到結構類似於脂質的離子對雙親分子decyltrimethylammonium-dodecylsulfate (DeTMA-DS, C10-C12)、decyltrimethylammonium-tetradecylsulfate (DeTMA-TS, C10-C14)作為主材料製備液胞，並經由半自發的製程，透過添加不同比例的乙醇(10-30 vol%)和膽固醇(XCHOL=0-0.5)，及pH值為7.4的緩衝溶液(Tris-HCl buffer)得到類乙醇體陰陽離子液胞分散液。接著以液胞作為藥物載體，包覆水溶性藥物(熊果素)，探討乙醇及膽固醇對其物理特性、藥物包覆行為之影響並與單一雙層膜液胞模型計算出的包覆效率理論值做比較，進一步探討液胞穩定性及水溶性藥物的包覆行為成功預測之關係。另外，並利用螢光非等向性(FA)方法來測定液胞雙層膜的堅硬度，以判別其作為經皮藥物傳輸載體之適用性。經實驗發現添加適當的乙醇濃度，則可以利於液胞形成，提升液胞穩定性，而添加膽固醇可以使液胞的穩定性更提升。並發現當添加適量的乙醇及膽固醇能使液胞穩定，其多分散指數(粒徑分布指數, Polydisperse Index, PDI)值小於0.3，則可使用單一雙層液胞模型成功準確預期水溶性藥物的包覆效率。此標準的建立可以使獲得包覆效率的數值更快速、便利，只需使用商業化的儀器動態雷射光散射儀 (Dynamic Light Scattering, DLS)確認所製備完成的液胞PDI值小於0.3，再將所測得的粒徑數值代入模型計算，即可獲得液胞的水溶性藥物包覆效率。同時利用螢光非等向性方法來測定液胞雙層膜的堅硬度，找出最適合作為經皮藥物傳輸載體的配方，而大部分適合作為經皮藥物傳輸載體的配方所製備的穩定的類乙醇體陰陽離子液胞，其雙層膜堅硬度相對於乙醇體(dipalmitoylphosphatidylcholine, DPPC)小，但會比乙醇體(Phospholipon 90 , PL-90)來的大，由於兩種乙醇體皆可運用於經皮傳輸上，因此類乙醇體陰陽離子液胞具有足夠軟的液胞雙層膜結構，雖然並非最軟，但也適合作為經皮藥物傳輸的載體。

Lipid-like catanionic surfactants have emerged as the attractive materials to prepare potential vesicular carriers in drug and gene delivery applications. In particular, the semi-spontaneous process has been developed to fabricate ethosome-like catanionic vesicles for the transdermal drug delivery. In this work, Arbutin (a water-soluble drug) encapsulation efficiency (E.E.) of ethosome-like catanionic vesicles fabricated from decyltrimethylammonium-dodecylsulfate (DeTMA-DS) and decyltrimethylammonium-tetradecylsulfate (DeTMA-TS) with various amounts of ethanol and cholesterol in tris buffer solution was experimentally determined. A simple unilamellar vesicle model, resulting in the theoretical E.E. within ±10% error for most vesicle compositions, was also developed. Stable vesicles with the aid of suitable amounts of ethanol and cholesterol, which led to polydispersity index (PDI) values less than 0.3, can be accurately predicted E.E. Moreover, bilayer rigidity was studied by fluorescence polarization technique. By examining ethanol and cholesterol effects on the fluorescence anisotropy (FA) of bilayers, possible applications for transdermal drug delivery carriers were evaluated. There may have been cholesterol formed cholesterol bilayer domain (CBD) by itself in bilayer which may have made vesicles unstable at high concentration of cholesterol. Therefore, the optimum formulations for transdermal delivery were excluded these compositions. For the optimum formulations, lower bilayer rigidity was exhibited as compared with that of dipalmitoylphosphatidylcholine (DPPC) ethosomes, but it exhibited higher bilayer rigidity than phospholipon 90 (PL-90) ethosomes. In short, ethosome-like catanionic vesicles for transdermal drug delivery was applicable.

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