本研究配合野外相關調查，評估水綿藻屬對營養鹽之攝取效果，進ㄧ步探討其應用於降低水庫或集水區氮磷濃度及改
善優養化現象之可行性。
前置實驗結果顯示，在培養基pH為9及不攪拌的情況下，水綿對營養鹽攝取效果較佳，應與其絮聚特性有關，培養週期
為4天，而在營養鹽攝取方面，其對碳酸鹽、硝酸鹽及磷酸鹽之最高攝取量，則分別可達12%、70%及25%。
批次實驗結果顯示，比較環境因子對單位藻體之總氮及正磷酸鹽攝取量的影響發現，在溫度、pH及營養鹽濃度皆為
high level的情況下水綿藻攝取效果較佳，其單位藻體對總氮與正磷酸鹽之攝取量最高分別可達36.89 mg/g．L與6.99
mg/g．L。經ANOVA轉換分析後，發現在總氮的部分並無顯著影響的單一或複合因子存在，而影響正磷酸鹽攝取之重要
影響因子主要是溫度及磷酸鹽添加濃度。
比較其對總氮之攝取實驗結果後發現，部分組別總氮量於實驗結果後卻有增加的情形，經增測TKN後發現，有機氮可能
藉由代謝作用而自藻體釋放至水樣中，其濃度為0-1.36 mg/g．L，故導致最終總氮變化量為負值。總脂質增加量最高
可達77.35%，且與水綿藻對氮磷攝取效果間具正相關性，意即此藻種不僅可降低水庫或集水區內氮磷含量，亦具有發
展生質能源之潛勢。
在藻種鑑定的部分發現，不同採樣地點之藻種S1、S2與S3，經16S rDNA鑑定後，皆屬於Zygnemataceae科中Spirogyra
屬，同集水區不同河道所存在之附著性藻類鑑定後並無明顯差異。

This research mainly combined with the field investigation to estimate the availability of assimilation
of nutrients by Spirogyra. The results could further apply to decrease the concentrations of nitrogen
and phosphorous and improve the eutrophication in the reservoir or watershed.
The results of primary experiments indicated the assimilated amounts of nutrients increased when the pH
of medium was 9 without stirring. It was probably attributed to the flocculated effects of Spirogyra.
The period of culture was 4 days and the highest assimilated amounts of carbonate, nitrate and
phosphorous were 12%, 70% and 25%, respectively.
The results of batch experiments showed the effects of total nitrogen and ortho-phosphate per dry
weight of Spirogyra with various environmental factors. The assimilated amounts by Spirogyra would
better when the pH, temperature and concentration of nutrients were classified to high levels. The
highest assimilated amounts of nitrogen and ortho-phosphate per dry weight of Spirogyra were 36.89 mg/g
．L and 6.99 mg/g．L. After transferring and analyzing with ANOVA software, we could find there was no
significant effect existed on the assimilation of total nitrogen. But the temperature and
concentrations of phosphate-adding were the major factors and had the apparent effects for assimilating
by Spirogyra.
To compare the results of assimilating amounts of total nitrogen we found that the values increased in
some experiments after cultivating. It was referred to the organic nitrogen released from algae via
metabolism function to induce the variations of total nitrogen to become negative and the
concentrations per dry weight of Spirogyra were about 0-1.36 mg/g．L. The total lipid contents
increased with increasing the assimilated amounts of nutrients and the highest value of raising was
77.35%. It was implied the Spirogyra could apply to decrease the concentrations of nitrogen and
phosphorous in the reservoir and watershed and also had the potential for developing bioresource.
We also used the molecular biology technique to identify the test algae and the results indicated the
S1, S2 and S3 could categorized as Spirogyra geuns and Zygnemataceae family. The different sampling
zones of watershed didn’t discover the variations in the types of attached algaes.

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