螢光共振能量轉移在此篇論文的錐狀光纖感測器的系統下被應用作標靶測量。多模錐狀光纖是採用熱熔拉法作製備，將其拉伸至錐狀區域最細處直徑為十微米以讓高模態的光逸出並與外界物質作接觸。接著再以(3-胺基丙基)三甲氧基矽烷之溶凝膠法對多模錐狀光纖的表面作胺基的改質，並探討 (3-胺基丙基)三甲氧基矽烷塗層的厚度對訊號偵測的影響，以得到更好的收訊。接著則會在經過胺基改質的光纖表面接上在螢光共振能量轉移裡扮演供體物質，一名為 Marina Blue 的螢光染料 (其激發波長為三百六十五奈米; 放光波長為四百六十五奈米)。再來則會引入一Y型的光纖藕合裝置以建立全光纖感測系統，其Y型構造的目的因為大部分的入射光源會在感測區作逸散並只有少部分能夠傳回系統內，從而降低入射光源對待測訊號的干擾。目標溶液的酸鹼度也經過調整以使帶有扮演螢光共振能量轉移受體 Chromeon 470 染料 (其激發波長為四百七十奈米; 放光波長為六百一十一奈米) 並具有羧酸化表面的奈米微粒能夠更容易地吸附上胺基改質的光纖表面。感測的進行是將經由Marina Blue染料改質的多模錐狀光纖浸入含有帶Chromeon 470 染料並具羧酸化表面的奈米微粒的溶夜裡，並觀察在由波長三百六十五奈米的入射光作內部激發所測得的螢光光譜裡經螢光共振能量轉移所激發的Chromeon 470的訊號。此錐狀光纖感測器的靈敏度為其偵測極限 9.655x105 個每微升至 1.105x105 個每微升，反應時間則為 9 至18 分鐘。

Förster resonance energy transfer (FRET) was introduced in the tapered optical fiber sensor for target detection. Multimode tapered optical fiber (MTOF) was obtained by heat-pulling method, and the diameter of its waist region was obtained to around 10 µm for the leak of higher-order modes light to make interaction with external substances. MTOF was further conducted by APS sol gel process in the aims of amino surface modification. The influence of APS coating thickness was also investigated for the better signal acquisition. Then, Marina blue fluorescence dye (λex: 365nm; λem: 465nm) which served as FRET donor would be conjugated on the amino modified fiber surface. A Y-shape combiner was adopted to construct the all fiber sensing system. The purpose of Y-shape design was to lower the interference of incident light, most of incident light would loss in sensing region and less would couple back. The pH value of target solutions were adjusted to obtain better adsorption of Chromeon 470 (λex: 470nm; λem: 611nm)-marked, surface carboxylated NPs which served as the FRET acceptor onto amino modified fiber surface. Detection was conducted by immersing Marina Blue functionalized MTOF into Chromeon 470-marked, surface carboxylated NPs contained solution and to observe the FRET induced Chromeon 470 signal in the fluorescent spectra collected via internal excitation by 365nm incident light. Sensitivity of the tapered optical fiber sensor is obtained with the detection limit of 9.655x105 /μl ~ 1.105x105 /μl, and the response time is within 9-18 min.

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