半導體奈米量子點是一種具有光致變色的嶄新材料，在生物醫學領域上常被廣泛的應用於螢光標定物及各種的生醫檢測，經由生物修飾後的奈米量子點可用於多種蛋白質追蹤與細胞行為長期的觀察。因此對奈米量子點被細胞攝入後，於胞內之行為表現與細胞胞器的相互作用值得作深入的探討。在研究中利用硒化鎘(核)包覆硫化鋅(殼)[CdSe/ZnS]此種奈米量子點，以兩種不同的物理機制，細胞自發性胞噬作用與電胞膜穿孔法將其送入老鼠成骨細胞 (MC3T3E-1)，進行觀察奈米量子點在不同機制下在細胞內的行為表現。
　　實驗的方法主要可分為三大方向，1、水相奈米量子點的製備，主要是利用化學氧化還原法進行合成具有親水性的奈米量子點，2、微型轉殖晶片製作，利用微機電製程技術在玻璃基材上製作出薄膜電極並利用高分子材料定義細胞反應區，3、生物奈米量子點傳遞觀察實驗，以電胞膜穿孔法與胞噬法在不同時間、濃度與緩衝液等參數調配下，將奈米量子點送入細胞，進行即時性細胞觀察與定性分析，研究中搭配不同的傳統染劑，比較兩者表現上的差異與觀察奈米量子點和細胞胞器結合的關聯性。偵測上，利用螢光顯微鏡、共軛焦顯微鏡及穿透式電子顯微鏡對所探討之樣本進行多項驗證，作為定性分析之交互比較，在論文中對於不同的樣品之前處理步驟也加以討論。
　　研究可歸類出以下幾點重要結果:其一成功合成水相奈米量子點，發現其對細胞轉殖作用性良好，可應用於生物系統光學追蹤，其二以電胞膜穿孔法將奈米量子點送入細胞內的數量高於胞噬作用，證明微型轉殖晶片具有高效率的穿孔效果，其三胞噬法與電胞膜穿孔法會造成奈米量子點傳輸機制與行為表現不同，第四點奈米量子點比傳統染劑更適合進行活體長期觀察與追蹤分析。第五點奈米量子點具有優良載體之特性，研究中發現它能經由兩種機制之輸送而進入細胞核內，因此我們藉由此項研究進一步了解奈米粒子與細胞作用的關係及可能應用的範圍，為生物奈米研究有了基礎的貢獻。

　　Semiconductor quantum dots (QDs) are a novel photochromic material. QDs have been applied to immunofluorescent labeling and various biological detection. With bioconjugated QDs, cells can be observed for long terms and multiple proteins can be traced. After the uptake of QDs, we discuss distribution of QDs inside cells and the interrelationship between organelles and QDs. In my study, we observe behavior of QDs inside cells under two kinds of uptake mechanisms-endocytosis and electroporation.
　　Three main topics: (1)Synthesis of hydrophilic QDs by using oxidation-reduction reaction; (2)Fabrication of micro Biochip, we employ MEMS(Micro Electro-Mechanical Systems) technique to make thin film electrodes on microslide and define the reaction area of cells by macromolecule material PDMS; (3)Observation of QDs inside cells, Under endocytosis and electroporation, with the adjustment of different time lapse points, concentration of QDs, and buffers, we make real time detection and qualitative analysis after the uptake of QDs. In comparison to organic dyes, we discuss behavior between QDs and dyes inside cells. By exercising fluorescent microscope, confocal microscope and Transmission Electron Microscope, we get some results for qualitative analysis and interaction. In this study, we also discuss pre-treatment for different samples.
　　We find some important results: (1)We successfully synthesize hydrophilic QDs and it is well applied to biosystem opitcal trace; (2)Transfection ratio of electroporation is better than that of endocytosis for QDs; (3)Different physical mechanisms have different communication and behavior between QDs and cells; (4)QDs are more suitable than organic dyes in long term observation and analysis trace; (5) We find QDs get into nucleus by endocytosis and electroporation, to prove that QDs own good vector characteristic. By this study, we further understand not only the relationship between nanoparticles and cells but also its applicable field.

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