目前主要危害台灣石斑魚養殖業的病毒以神經壞死病毒以及虹彩病毒為主。屬於虹彩病毒科的巨大細胞屬病毒會造成魚隻器官腫大以及死亡，所以要了解病毒的機轉以及找到防治的方法，以儘快解決此問題，也是本論文主要之研究目的。本論文利用傳染性脾臟及腎臟壞死病毒(ISKNV)來感染宿主GF-1細胞，在此過程中發現病毒感染會誘導ROS上升，並增加促凋亡基因Bax、Bak以及tBid增加表現，降低抗凋亡基因Bcl-2及Bcl-xL表現，增加粒線體膜電位喪失，活化caspase家族如caspase-8、caspase-9以及caspase-3，最後造成細胞凋亡。添加抗氧化劑可以降低ROS，並降低促凋亡基因表現，影響抗凋亡基因表現，降低粒線體膜電位喪失，抑制caspase 家族活化，可以增加細胞存活率。另一方面可以降低抗氧化酵素SOD和catalase表現，可以調節氧化壓力平衡。另外發現添加抗氧化劑可以降低病毒複製。總結以上結果，病毒感染可以藉由誘導ROS上升來調節促凋亡基因表現造成細胞凋亡，而添加抗氧化劑則可以抑制此結果，並調節氧化壓力平衡，降低病毒複製。

So far main viruses, nervous necrosis virus (NNV) and iridovirus, endanger grouper aquaculture in Taiwan. Infected fishes by megalocytivirus belonging to family Iridoviridae appear organomegaly and cause death, so understand the mechanism of viral infection and finding prevention methods to solve this problem, and also the major studied propose in this thesis. In this thesis, the use of infectious spleen and kidney necrosis virus (ISKNV) for the study, to infect GF-1 cell, in the process to find within this infection that increasing ROS could increase pro-apoptotic genes such as Bax, Bak, and tBid, and reduce anti-apoptotic Bcl-2 and Bcl-xL, thus enhance mitochondrial membrane potential (MMP) loss, and activate caspase family such as caspase-8, caspase-9 and caspase-3, so finally cause cell death. Antioxidants treatment could reduce expression of pro-apoptotic genes, affect expression of anti-apoptotic gene, reduce MMP loss, and inhibit activation of caspase family, finally increase cell viability. On the other hand, they could reduce anti-oxidative enzymes SOD and catalase and inhibit viral replication. In sum, viral infection could induce ROS increasing to regulate expression of pro-apoptotic genes causing cell death, and antioxidants treatment could inhibit this result, regulates the balance of oxidative stress, and reduces viral replication.

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