多光子時域聚焦顯微術(temporal focusing multiphoton microscopy，TFMPM)利用超快雷射脈衝能量的集中特性，以較低的平均功率達成高瞬時功率進行樣品激發，減少雷射高能量對樣品的傷害，而TFMPM系統搭配於線掃描的掃描方式能夠快速地進行樣品激發掃描，實現三維螢光影像重建，本文將與適應性光學系統(adaptive optics，AO)結合，進行脈衝寬度的補償與色散特性的應用。
本文首先將建立一套AO系統，利用Zernike多項式做為數學模型，搭配本實驗室自製波前感測器(Shack-Hartmann wavefront sensor，SHWS)進行波前偵測與可調變式聚焦鏡(deformable mirror，DM)做為前修正器，並透過三步系統建模優化獲得最佳像差模態，最後利用單獨調控像差特性進行靜態干擾修正與遙控調焦之應用，並利用此技術結合TFMPM系統，架設干涉式自相關(fringe-resolved autocorrelation，FRAC)量測脈衝寬度，並利用液態透鏡改變面形進行TFMPM的遙控調焦，最後將架設一套適應性脈衝壓縮系統，利用DM進行色散的補償達成螢光強度的提升。

Temporal focusing multiphoton microscopy (TFMPM) uses the concentration of ultrafast laser pulses to get high power for sample excitation at low average power, reducing laser-induced damage to the sample. Using a line-scanning method, the TFMPM system allows fast sample excitation and scanning, enabling three-dimensional fluorescence imaging reconstruction. This study aims to add adaptive optics (AO) to the system to adjust pulse width and control dispersion.
First, we will establish an AO system using Zernike polynomials as the mathematical model, combined with a custom-built Shack-Hartmann wavefront sensor (SHWS) for wavefront detection and a deformable mirror (DM) for wavefront correction. Through a three-step system modeling process, we will optimize and obtain the high-contrast aberration modes. Finally, by independently adjusting aberration characteristics, we will perform static disturbance correction and remote focusing applications. This technology will be integrated with the TFMPM system, setting up fringe-resolved autocorrelation (FRAC) to measure pulse width, and using a liquid lens to adjust the surface shape for TFMPM remote focusing. Ultimately, an adaptive pulse compression system will be established, using the DM to compensate for dispersion, thereby enhancing fluorescence intensity.

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