本文研究目的在發展胞解微型晶片技術取代傳統水浴法時效冗長之缺點，以微機電製程製作之原型胞解晶片經即時聚合酶偵測系統的定量分析實驗，證實效率低於傳統方式，為了解影響胞解效率之參數與晶片改進方向，決定利用數值分析法建構模型進行快速胞解微晶片之熱流場分析，採用CFD-RC工程分析軟體作為分析工具，藉以建立電場、流體與熱傳三種物理量之耦合模式進行研究，胞解熱酵素法反應機制中以microLYSIS作為胞解酵素，反應首次週期為96C、2分鐘之後降至65C、4分鐘，爾後進入第二週期，反應時間因酵素動力學而逐次遞減，常態下以三週期為基準，因此晶片的設計必須在能量與流速之間取得最佳平衡關係，經過CFD-RC的耦合分析，發現原型實驗晶片僅有第三週期達到所望溫度，此結果可以說明晶片胞解效果不佳之原因，研究中發現電極幾何形狀、流速與流道高度為影響反應溫程之重要參數，經由參數最佳化模擬結果得知，以電壓0.6V、流速0.1mm/s以下，三週期溫程可達到胞解環境，而第二型晶片增加預熱電極之設計，不僅降低工作電壓至0.49V，流速可提高至0.2mm/s，暫態效果耦合場分析，更能精確計算出加熱電極之溫程變化情形，作為後續製程改善之依據。

　　This study was focusing on developing microchips for rapid DNA cell lysis to replace the traditional method that has the disadvantage in long time process. The prototype lysis chip was fabricated by microfabrication technology and verified using a real time PCR system. The lysis efficiency using the lysis chip was lower than that of the conventional method. In order to improve the design and understand the parameters which affected the lysis efficiency, CFD-RC software was used to understand the temperature distribution by analyzing the electric-thermal-fluid coupled-fields. The conventional cell lysis process used lysis enzyme (microLYSIS) under the thermal cycle at 96C, 2 min and 65C, 4 min for three cycles. The lysis chip was designed in flowing through and passing different temperature zones to achieve three thermal cycles. So, to find the appropriate mixture injection velocity is necessary. By using CFD-RC coupled-field analysis to simulate the prototype chip, the results showed that the efficiency was low due to that only the third cycle could approach lysis temperature. The parameters which affected the lysis efficiency were electrode geometry, injecting velocity and microchannel’s height. When applying 0.6 voltage with 0.1 mm/s of injecting velocity, the simulation data showed all thermal cycles reached lysis temperature requirement. For the second type of chip, the preheating electrodes were added, therefore, the applied voltage was reduced to 0.49V and increased the injection velocity to 0.2 mm/s. The coupled-field analysis can provide a detailed information in designing the lysis chip for further improvement in efficiency.

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