微藻的生長速率快且可經由光合作用固定大氣中的二氧化碳並轉化生成生物質，是相當具有潛力的生質能源生產者。因此，本研究為提高微藻生物產量及其二氧化碳利用效能，設計複合型光生物反應器以增加碳源質傳效率，並探討最佳的氣體供應條件，再透過模型的建立決定出複合型系統中最佳的操作體積條件。
本研究在吸收塔與培養槽體積皆為2 L的複合型光生物反應器中，以光強度為600 μmol/m2s、氮源濃度為0.1 g/L的條件進行培養，探討不同的二氧化碳氣體流量及空氣流量(循環流率)之下，小球藻的生長表現。而最佳的氣體供應條件為：2 L/min的空氣流量與20 ml/min的二氧化碳混合之氣體，可得到最佳的產量及二氧化碳利用效率，分別為3.352 g/reactor及2.53 %。
由於擴大培養槽體積會提高培養系統的光照時間比例，且上述小體積培養之光照時間比例(12 hr/day)偏低，不利於進行模擬及預測，故以培養槽體積擴增為7.5 L之複合系統進行培養，其吸收塔之體積及通氣條件為二氧化碳濃度2 %的混合氣體以2 L/min的流量通入2 L的吸收塔。以Logistic方程式及PFR之原理描述小球藻的生長及在吸收塔中二氧化碳的吸收情形，並以培養槽體積為7.5 L之實驗數據進行擬合。藉由此模型的建立決定出最佳的培養槽體積，並透過實驗驗證而得到判定係數值為0.97。最佳的培養槽體積為26 L，經實際培養得到比生長速率及最終生物產量分別為0.0345 hr-1和30.6 g/reactor，且其碳源利用效率可提升至14 %左右。因此證實複合型光生物反應器可有效提升微藻產量以及二氧化碳利用效能，而模型可有效掌握複合系統中小球藻的生長情形。

Microalgae have received much attention as renewable energy resources, since the photoautotrophic mechanism can convert the atmospheric carbon dioxide into biomass. In order to harvest microalgae with the high production of biomass and provide high carbon dioxide utilization efficiency (CUE), the hybrid type photobioreactor was developed to improve the carbon dioxide mass transfer efficiency in the cultural system. After finding out the optimal gas supply conditions by experiments, a model was built up to obtain the optimal volume of cultural tank.
In this study, different aeration rates of carbon dioxide and air were carried out in the hybrid system, which was composed of 2 L absorption tower and 2 L cultural tank under 600 μmol/m2s of light intensity and 0.1 g/L of initial urea concentration. The best condition for obtaining large biomass production and high CUE was aerated with 2 L/min air and 20 ml/min carbon dioxide (2 % CO2), and there were 3.352 g biomass/reactor and 2.53 % of CUE from the batch cultivation.
It seems that increasing the cultural tank volume without any changes of the conditions in absorption tower can enhance the lighting hours of cultural system. The lighting hours in the hybrid system mentioned above (about 12 hr/day) was too short to use for model, so a 7.5 L-cutural tank of hybrid system, which has 19 hr/day of lighting, was carried out. For knowing the growth kinetics of Chlorella sp. and the consumption of carbon dioxide, Logistic equation and the principle of plug flow reactor (PFR) are considered as the model to approach the dynamics and mechanism. The optimal volume of cultural tank was 26 L, which was determined by model, with 2 L of absorption tower, which is aerated by 2 % CO2, and the biomass production, specific growth rate and CUE are 30.6 g/reactor, 0.0345 hr-1 and about 14 %, respectively. Therefore, the hybrid type photobioreactor is successfully employed for enhancing the production of biomass and carbon dioxide utilization efficiency.

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