傳染性疾病可由其病原體特定DNA或蛋白質來辨識。拉曼光譜以雷射光與檢測分子交互作用後直接分析分子結構與官能基進而提供指紋性圖譜。拉曼光譜更可結合SERS效應提高其檢測靈敏度，非常適合應用於生醫檢測上。本研究使用「空間強化型SERS活性基板」結合微流道建立以拉曼光譜方式辨識病原體特定核酸序列之檢測平台。
利用奈米壓痕試驗機可在單一步驟下製作出精密度高之特異性孔洞結構陣列，並透過控制壓痕間距來縮減奈米孔洞開口大小進而提高深寬比，以此強化表面電漿子侷限化之作用進而產生較大之表面增顯拉曼散射效果。本研究選用禽流感病毒H7特殊核酸片段進行不同雜合狀態拉曼光譜檢測。在低濃度下，利用互補序列作為捕捉與篩檢探針於懸浮狀態及固定狀態下進行檢測，並藉由分析指紋性圖譜來辨識是否與目標待測物產生雜合。進一步將空間強化型奈米孔洞結構整合於微流道中藉由比對拉曼光譜對不同核酸序列進行免標定檢測及辨識。
研究成果顯示：以空間強化型SERS活性基板針對禽流感H7-Target核酸片段及互補序列H7-Probe進行免標定之拉曼光譜檢測，能夠完整地呈現之指紋特性拉曼圖譜且檢測濃度極限可達10-10 M，且由拉曼圖譜比對以738 cm-1峰值的消失及相對最高峰由1603 cm-1位移至1554 cm-1來辨識H7核酸片段是否產生雜合。藉由將H7-Probe改質於基板上並作為捕捉端並整合於微流道中可成功辨識互補核酸序列及非互補核酸序列。以上成果證實可應用於早期病毒病原體檢測及辨識用途。

Infectious disease is recognized by the pathogens biomarker identification of specific DNA or proteins. Raman spectroscopy is a technique used to analysis molecule structure and provide the specific spectra by the interaction between the molecule and laser light. The detection sensitivity of Raman spectra can be induced by SERS, so it is good for Biomolecules analysis. In this study, we developed a detection method for recognizing biomarker, such as specific DNA sequence by SERS spectra with combining spatially reinforced Au nano-cavities (SR-nAu) substrate and micro-fluidic channel.
In this study, we used Nano-indentater, by controlling the tip-to-tip displacements to reduce the volume, to fabricate SR-nAu substrate for detecting specific DNA sequences of Avian influenza with virus hybridization responses; In the nano-cavity, suspended, or immobilized probe and its complementary target and mismatch sequences at very low concentration were characterized through enhanced Raman spectra. Furthermore, we confirmed SR-nAu in micro-fluidic channel to detect and recognize the different DNA sequences without labeling by Raman spectra.
These results demonstrated SR-nAu substrate was competent to recognize the various hybridization responses of H7 sequence by the presence of Raman shift at 738 cm-1 and the change of relative peaks intensity at 1603 and 1554 cm-1 before/after hybridization. By immobilizing H7-Probe on SR-nAu substrate as a capture site could sieve its complementary target and mismatch sequences. Based on these result, the development of detect platform could be applied to early bio-medical detection.

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