核醣體蛋白(Ribosomal protein)主要功能為穩定rRNA的結構與促進rRNA行正確的折疊並參與在核醣體生合成中，根據核醣體蛋白組成的不同讓核醣體選擇性地轉譯特定核糖核酸。核醣體蛋白L19(RPL19)為核醣體大次單元中的一員，在前列腺癌與大腸直腸癌的中被當作是一預後指標，RPL19的改變不僅可以影響前列腺癌癌細胞侵略性表現更是影響轉譯作用的精確度。儘管有許多關於RPL19的功能被報導，但是RPL19如何參與在轉譯調節中的機制尚未被研究透徹。本篇研究中主要利用建構在高通量定序之上的核醣體定位技術來探討在RPL19存在、表現量下降的系統中基因的表現改變。我們發現表現量顯著下降的基因其功能主要參與在細胞週期的合成期(Synthesis phase)。同時，也觀察到5' 端帽結合蛋白 eIF4E表現量下降，可能會導致進行5’端帽起始轉譯的數量減少。結合核醣體內部結合位預測軟體的使用，挑選出多個基因深入探討RPL19的調節機制。進一步將這些基因的五端非轉譯區(5’UTR)序列建構出來，後續再以報導基因分析釐清RPL19是否透過結合上核醣體內部結合位區域來調節蛋白質的生合成。

Ribosomal protein L19 has been reported to be a prognostic marker for prostate cancer as well as colorectal cancer. Alteration of RPL19 would decrease the fidelity of translation and abrogate aggressive phenotype of cancer. Nontheless, the underlying mechanism of RPL19 participating in global genome mRNA translation is not clear. In the study, we used ribosome profiling (Ribo-seq) technology to investigate the genome-wide translation change between the normal and RPL19 decrease condition. We find that the significantly down-regulated genes mainly involving in the S phase of cell cycle. Moreover, we also found the down-regulation of eIF4E ,a cap-binding protein, play a key role in switching the translation initiation mechanism. According to RPF result, we proposed that IRES dependent translation is favourable under RPL19 declined. Combined with the IRES prediction database, few S-phase associated genes were selected. Furthermore, the reporter assay of 5’UTR of selected genes were constructed to clarify whether RPL19 interact with IRES element to control protein synthesis.

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