分子模版高分子 (Molecularly imprinted polymers；MIP) 乃是藉以欲分析物或稱模版分子 (template) 先與功能性單體 (functional monomer) 形成以鍵結作用力吸引的複合物型態；聚合時，單體圍繞模版分子，同時加入交聯劑 (crosslinker) 將模版分子固定化。經溶劑萃取方式將高分子內之模版分子洗出，以形成多孔性可辨識區域，即可製備出對欲分析物具有優異親和性及特異選擇性之高分子。
本實驗以判定黃疸之指標性分子，膽紅素作為模版分子，以甲基丙烯酸 (Methacrylic acid；MAA) 及乙二醇二甲基丙烯酸酯 (ethyleneglycol dimethacrylate；EGDMA) 分別作為功能性單體及交聯劑，並加入偶氮異二丁腈 (2,2’-azo-bisisobutyronitrile；AIBN) 起始劑進行熱聚合。製備完成之聚合物以EDTA/NaOH萃洗液萃取包埋於內之膽紅素模版分子，以形成具有辨識膽紅素之孔洞。並藉由比表面積測定 (Brunauer-Emmett-Teller analyzer；BET)、掃瞄式電子顯微鏡 (scanning electron microscope；SEM) 分別進行膽紅素模版高分子之物性測定與表面觀察。膽紅素分子極易因光和氧氣而氧化，故首先探討EDTA/NaOH背景溶液及膽紅素溶液之穩定性。
本研究亦針對膽紅素模版高分子於單成份與多成份混合溶液系統中對膽紅素之吸附選擇性進行討論；並對MIP於單成份及雙成份混合溶液中之吸附動力予以探討，由此進一步建立吸附模式，求取其模式參數並予以討論。除此之外，亦探討加入鹽類對MIP吸附膽紅素之影響。MIP於多成份混合溶液系統下對膽紅素及共存物 (黃體酮、睪固酮、膽固醇) 之吸附選擇性亦予以討論，並延伸多成份系統至真實血清溶液中，且建立MIP對膽紅素之吸附檢量線。

Molecularly imprinted polymer (MIP) was prepared by the interaction between the analyte or the template molecule and the functional monomer. During the polymerization, the cross-linker was added to confine the template molecule. Later, the template bound within the MIP was extracted by the utilization of solvent, the cavities as the recognition sites were thus formed. In this way, the MIP being made can have superior affinity and specificity towards the analyte.
In this research, bilirubin, being the index for jaundice, was used as the template molecule. Methacrylic acid (MAA) and ethyleneglycol dimethacrylate (EGDMA) were used as the functional monomer and cross-linker, respectively. 2,2’-azo-bisisobutyronitrile (AIBN) was added to initiate the thermal polymerization. The MIP thus prepared was then washed by EDTA/NaOH solution for the extraction of bilirubin template molecules. Specific surface area analysis (Brunauer-Emmett-Teller analyzer; BET) and scanning electron microscope (SEM) were used to measure MIP’s physical properties as well as to observe its surface morphology. How the EDTA/NaOH solution affected the stability of bilirubin was investigated. The binding specificities of the MIP in the single solution and binary mixture were compared and discussed. The adsorption isotherm as well as the kinetic model was established. The parameters in the model were obtained and compared. The influence of salt for the adsorption performance was also discussed. The binding specificity of the MIP in triple mixture solution was further investigated. In addition, the serum samples were prepared to establish the calibration of bilirubin concentration via the adsorption of the MIPs.

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