點帶石斑魚 (Epinephelus coioides) 為台灣重要經濟魚種之一。近年來受到神經壞死病毒 (nervous necrosis virus, NNV) 之影響，造成魚苗死亡率居高不下。根據本實驗室先前研究結果顯示，點帶石斑魚受到神經壞死病毒感染時會誘導干擾素 (interferon, IFN) 相關免疫基因表現，啟動一連串防禦機制以保護魚體。因此為探討干擾素在抵禦病毒入侵所扮演之機制，在本研究中已選殖出點帶石斑魚干擾素 (orange-spotted grouper IFN, osgIFN) 序列，並以感染神經壞死病毒之點帶石斑魚為試驗材料，探討osgIFN與病毒之交互作用。從實驗結果觀察到在不同病毒感染時間下，osgIFN表現量有伴隨時間增加而有上升之趨勢。另外，在探討抗病毒活性的部分，也已證明 osgIFN 確實能夠誘導 Mx 基因表現，並且進一步觀察到此現象具有組織特異性，尤其在免疫器官及神經系統對於干擾素感受性特別強烈。最後，本研究證實osgIFN具有分泌活性，在魚體受到病毒感染後會被誘導表現，並進一步分泌到細胞外，促使鄰近細胞產生抗病毒活性。本研究成果已可初步解釋osgIFN在病毒感染情況下的反應情形，以及所誘發之相關免疫機制，對於往後點帶石斑魚養殖產業之神經壞死病毒防治上或具有參考價值。

Grouper aquaculture industry is considered as one of a potential economic industries in Taiwan, and orange-spotted grouper (Epinephelus coioides) is the main breeding species. However, in recent years, nervous necrosis virus (NNV) leads to high mortality rate, and cause serious crop losses of fishermen. According to previous studies in our laboratory, virus infection will turn on immune gene to protect the fish, which mostly induced by interferon (IFN). IFN was induced by virus infection to open the downstream antiviral mechanism, therefore, this study will invesgate the IFN expression in different post infection time and organs, to realize the activation of antiviral protein. In here, we cloned the sequence first. OsgIFN contain 769 base pairs, encoding 172 amino acid and one signal peptide. Classfication of osgIFN belongs to 2C-IFN. According to the real-time PCR results, osgIFN mRNA level increase with the infection time, and the significantly increasing trend was observed in immune organ like head kidney and intestine. On the other hand, IFN was know to be screated cytokine, the gene will turn on after virus infection, and then the IFN protein secreated from cell to cell, leads the neighboring cells induce antiviral protein. Therefore, at the part of antiviral activity investigation, osgIFN recombinant protein treatment will promote Mx promoter activity in GF-1, shows that osgIFN can actually induced Mx gene. At the part of tissue distribution analysis of Mx gene induced by osgIFN, the results from real-time PCR show that there are significantly increasing trend in head kidney and eye, indicating that the susceptibility is higher in the immune organ and nervous system.

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