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研究生：	高維毅 Kao, Wei-Yi
論文名稱：	玻尿酸醱酵中均衡培養基之探討 Study on Balanced Medium in Hyaluronic Acid Fermentation
指導教授：	陳特良 Chen, Ｔeh-Liang
學位類別：	碩士 Master
系所名稱：	工學院 - 化學工程學系 Department of Chemical Engineering
論文出版年：	2006
畢業學年度：	94
語文別：	中文
論文頁數：	83
中文關鍵詞：	醱酵、玻尿酸、鏈球菌、均衡培養基
外文關鍵詞：	streptococcus, hyaluronic acid, fermentation, balanced medium
相關次數：	點閱：95 下載：1
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在本論文中以Streptococcus zooepidemicus ATCC 39920醱酵生產玻尿酸時，葡萄糖和酵母萃取物養分濃度比例為2.5是醱酵的均衡培養基。當葡萄糖和酵母萃取物的比例大於2.5時，殘留的葡萄糖會轉換成為有機酸而不會轉換成菌體或玻尿酸；而當氮源的添加量過多時，大多數的葡萄糖會被使用來提供細胞生長而不用來生產玻尿酸。饋料批次培養的結果顯示當碳源或氮源成為限制基質的時候，是不適合用來生產玻尿酸。綜合以上的結果，玻尿酸的生產應以均衡培養基來進行醱酵。

The balanced medium for producing hyaluronic acid by Streptococcus zooepidemicus ATCC 39920 was composed of glucose and yeast extract with a ratio of 2.5 in this study. When the ratio of glucose to yeast extract was higher than 2.5, the residual glucose transformed to organic acid rather than biomass or hyaluronic acid. When the nitrogen source was excess, most of the glucose was used to support cell growth rather than hyaluronic acid production. The fed-batch culture showed that either carbon or nitrogen source becoming the limiting substrate was not favorable for the production of hyaluronic acid. Based on the above finding, the production of hyaluronic acid should be performed with a balanced medium.

目錄
中文摘要...............................................................................................................................Ⅰ
英文摘要...............................................................................................................................Ⅱ
誌謝.......................................................................................................................................Ⅲ
目錄.......................................................................................................................................Ⅳ
表目錄...................................................................................................................................Ⅶ
圖目錄...................................................................................................................................Ⅷ

第一章 緒論............................................................................................................................1
1-1 前言...................................................................................................................................1
1-2 玻尿酸的來源...................................................................................................................2
1-3 玻尿酸的結構...................................................................................................................3
1-3.1 一級結構........................................................................................................................3
1-3.2 二級結構........................................................................................................................3
1-3.3 三級結構........................................................................................................................6
1-4 玻尿酸生化合成途徑.......................................................................................................6
1-5 玻尿酸的應用...................................................................................................................9
1-5.1 玻尿酸在醫藥上的應用................................................................................................9
1-5.2 玻尿酸在化妝品上的應用..........................................................................................12
1-5.3 玻尿酸在保健食品上的應用......................................................................................13
1-6 玻尿酸衍生物的應用.....................................................................................................13
1-6.1 Hylans...........................................................................................................................14
1-6.2 HA Esters......................................................................................................................15
1-7 文獻回顧與研究動機.....................................................................................................16
第二章 實驗材料與方法......................................................................................................18
2-1 實驗菌株及實驗藥品.....................................................................................................18
2-1.1 實驗菌株......................................................................................................................18
2-1.2 實驗藥品......................................................................................................................18
2-2 實驗儀器與實驗裝置.....................................................................................................19
2-2.1 實驗儀器......................................................................................................................19
2-2.2 實驗裝置......................................................................................................................21
2-3 培養基的組成.................................................................................................................21
2-3.1 前培養..........................................................................................................................21
2-3.2 批次(Batch)培養...........................................................................................................21
2-3.3 饋料批次(Fed-Batch)培養............................................................................................25
2-4 實驗方法.........................................................................................................................26
2-4.1 菌株保存......................................................................................................................26
2-4.2 菌株活化......................................................................................................................27
2-4.3 前培養..........................................................................................................................27
2-4.4 主醱酵實驗..................................................................................................................27
2-5 實驗流程.........................................................................................................................27
2-5.1 批次醱酵實驗..............................................................................................................27
2-5.2 饋料批次醱酵實驗......................................................................................................28
2-6 分析方法.........................................................................................................................32
2-6.1 菌體濃度分析方法......................................................................................................32
2-6.2 玻尿酸濃度分析方法..................................................................................................36
2-6.3 葡萄糖濃度分析方法..................................................................................................39
第三章 結果與討論...............................................................................................................41
3-1 批次醱酵實驗中均衡培養基之探討..............................................................................45
3-2 氮源為限制基質時，對菌體生長和玻尿酸生產的影響..............................................61
3-3 碳源為限制基質時，對菌體生長和玻尿酸生產的影響..............................................69
第四章 結論...........................................................................................................................78
參考文獻................................................................................................................................79
自述........................................................................................................................................83

                                    

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