本研究利用四種離子對雙親分子為材料，分別為DeTMA-DS (decyltrimethylammonium-dodecylsulfate)、DeTMA-TS (decyltrimethylammonium-tetradecylsulfate) 、DTMA-DS (dodecyltrimethylammonium-dodecylsulfate) 和HTMA-DS (hexadecyltrimethylammonium-dodecylsulfate)，以半自發製程製備穩定的類乙醇體陰陽離子液胞(ethosome-like catanionic vesicles)。並添加疏水性螢光探測分子DPH(1,6-diphenyl-1,3,5-hexatriene)，運用螢光偏極化偵測，探討乙醇與膽固醇濃度、溫度對類乙醇體陰陽離子液胞雙層膜流動性的影響。
實驗結果顯示，在四個不同溫度下測量，固定膽固醇濃度下，乙醇濃度的增加，會造成雙層膜的流動性上升。推測可能的原因是，乙醇分子嵌入離子對雙親分子頭基，撐開離子對雙親分子之間的距離，使得雙層膜中碳氫鏈自由度增加，流動性提升。固定乙醇濃度下，膽固醇濃度增加，對DeTMA-DS與DeTMA-TS液胞系統，結果顯示雙層膜流動性減小；但對於DTMA-DS與HTMA-DS的液胞系統，雙層膜的流動性變化並不顯著。推測膽固醇在碳鏈較短的系統，由於膽固醇氫氧基產生的氫鍵作用力，以及膽固醇固環的限制，所以呈現膽固醇凝縮效應；而在碳鏈較長或對稱的系統，則又由於膽固醇尾端碳氫鏈干擾，膽固醇對雙層膜流動性的影響就不太顯著。在溫度效應部份，溫度上升對雙層膜流動性影響並不顯著。推測原因是，碳氫鏈一方面因為溫度的提升，流動性上升，但是另一方面，雙層膜因為產生指插現象，造成粒徑明顯下降，使得流動性減小，流動性隨溫度變化因而變得不顯著。

In this work, fluorescence polarization techniques were used to study the additives and temperature effects on the rigidity of vesicular bilayers membrane, which play crucial roles in determining drug encapsulation and release of vesicles. Four stable ethosome-like catanionic vesicles for the transdermal delivery of drug were fabricated by using four catanionic surfactants (or ion-pair amphiphiles, IPAs), different amount of ethanol, and various concentration of cholesterol in water solution through a simple semispontaneous process.
The experimental results showed that increasing concentration of ethanol did fluidize the bilayers rigidity under four different temperature. It was resulted from enlarging space between ion pair amphiphiles by ethanol inserting into headgroup. Bilayers rigidity of two vesicle systems increased with the increase of cholesterol concentration, while bilayer rigidity of the other two vesicle systems presented unapparent variation with the increase of cholesterol concentration. One explanation of the cholesterol ordering effects was restriction by both cholesterol hydrogen bonding and rigid ring. The other one of unobvious cholesterol effect was fluidization by cholesterol hydrocarbon tails besides.
With raising temperature, bilayers rigidity did not present obvious drop. On one hand, fluidity of hydrocarbon chains increased with raising temperature. On the other hand, it found that vesicles size of all four vesicle systems decreased with raising temperature., It would turn into interdigitated phase, and so that bilayers rigidity increase then. As a result, temperature effect on membrane fluidity became unobvious.

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