蒙脫土(Montmorillonite)為天然矽酸鹽礦產，常被作為載體，被應用的領域很廣泛，近年來已成為抗菌材料良好的基材。本研究針對經界面活性劑泊洛沙姆(poloxamer)改質的市售蒙脫土進行材料分析以及對大腸桿菌(Escherichia coli)抗菌活性檢測，有助於對此改質黏土有基本了解，進一步提出其相關可能的抗菌機制。
利用電子顯微鏡(SEM)、X光繞射儀(XRD)、紅外光譜儀(FTIR)、示熱差分析儀(DTA/TG)、X光電子能譜儀(XPS)和感應耦合電漿質譜儀(ICP-MS)等，進行材料結構與組成分析。實驗結果得知改質蒙脫土呈現層狀結構，主要由鈣、鎂、鋁、矽、以及氧元素等所組成，其中介面活性劑則占總重百分之二十四。接著評估改質蒙脫土對大腸桿菌抗菌活性，發現當其劑量高達2310 ppm時，其釋出鈣離子濃度為110 ppm，已顯著抑制細菌生長。除此之外，為了瞭解抗菌機制源由，本研究一方面收集了經過抗菌活性測試後的黏土，重新植菌，評估其抗菌能力；另一方面，比較抗菌前後其材料的差異性。結果顯示使用後的黏土無法再具有抗菌能力，且液態培養基中含有黏土所釋出的鈣離子與泊洛沙姆的濃度隨之顯著下降了，從FTIR、XPS、和ICP-MS實驗結果得到一致性變化趨勢，加以佐證。
根據結果推論，此改質蒙脫土的抗菌機制可能與黏土本身釋出鈣離子與泊洛沙姆有密切關係，並且蒙脫土因良好吸附能力而引起抗菌加成作用，而達到良好的抗菌效果。

Montmorillonite(MMT) is the natural aluminosilicate clay mineral. It has been widely applied in various fields, especially used as antibacterial agent carriers. The purpose of this study was to characterize commercial poloxamer-modified montmorillonite clay (PM-MMT) and to estimate its antibacterial activity against Escherichia coli (E. coli). Furthermore, the possible antibacterial mechanism of PM-MMT clay will be proposed.
First of all, PM-MMT clay was characterized with its morphology and compositions using scanning electron microscopy (SEM), X-ray diffractometry (XRD), Fourier transform infrared (FTIR), differential thermal analysis/thermogravimetric analysis (DTA/TG), X-ray photoelectron spectrometer (XPS), and inductive coupled plasma-mass spectrometer(ICP-MS). It showed that PM-MMT has the sheet structure, mainly composed of Ca, Mg, Al, Si and O elements in clay, and a weight fraction of around 24% poloxamer. Second, E. coli was chosen as an indicator to estimate the antibacterial activity of the PM- MMT clay. It was found that E. coli with the treatment of 2310 ppm clay was depressed their growth, and clay released calcium ion around 110 ppm. Besides, in order to know if the PM-MMT clay exhibited antibacterial activities, the used clay after antibacterial assys was collected, estimated their antibacterial activities, and compared the differences of the compositions. However, the used clay no longer retained antibacterial activities anymore. From FTIR, XPS and ICP-MS consistent results, the released amount of calcium ion and poloxamer from used caly dramatically decreased.
According to the above results, the possible antibacterial mechanism may relate to the release of the poloxamer and calcium ions. In addition, PM-MMT can achieve good antibacterial effect due to the synergistic action of adsorption capability

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陳自強，氫化物生成技術結合動態反應管感應偶合電漿質譜儀於生物及合金樣品中鎵、鍺、砷、硒、銻之分析，國立中山大學化學研究所，2005。