本研究中，交聯鍵結反應的多電層組裝修飾可在聚雙甲基矽氧烷基材之表面提供長效親和性修飾及產生與蛋白質作鍵結的活性官能基。聚雙甲基矽氧烷所製的免疫酵素分析微量盤是以此方式製造的。接著，在此微量盤上，使用免疫酵素分析法定量乳癌細胞與肺癌細胞細胞萃取液中的微量蛋白質。
使用三明治法，此分析系統具有偵測極限為5 pg/μL 。以連續稀釋樣品的偵測得知對乳癌細胞萃取液的動態範圍為41 到 12 pg/μL。所得檢量線為Y=0.0018X+0.022，R2=0.9956，線性範圍在277到 17 pg/μL。在乳癌細胞萃取液測得雌激素接受器α的濃度為41 ± 5 pg/μL(Mean ± SD)。然而，在肺癌細胞萃取液因太少無法測得。由添加樣品的偵測得知，在乳癌細胞與肺癌細胞細胞萃取液中，雌激素接受器α的回收率為102 ± 4 及 94 ± 5 %(Mean ± SD)。
由檢量線的再現性得知此微量盤的長期穩定性為5天。相較於市售品，此微量盤具有較長的開封後保存期限。
更進一步，微小化的此微量盤可經由影像擷取系統，以三明治方法，在乳癌細胞萃取液中，能辨識出肌凝蛋白與雌激素接受器α，此方法的優點在於降低樣品耗量與即時偵測。

In this research, the polyelectrolyte assembly modification with the cross-linking reaction can provide the long-term affinity modification and create the active function group for the protein binding on the PDMS substrate. The PDMS ELISA plate is fabricated by this method. Then, the low abundance proteins in the MCF7 and A549 cell lysates are quantified by the enzyme-linked immunosorbent assay (ELISA) method on this plate.
In the sandwich method, this assay system has the detection limit with 5 pg/μL and the dynamic range from 41 to 12 pg/μL for the MCF7 cell lysate in the detection of the serial dilution sample. The calibration curve is estimated to be Y=0.0018X+0.022 with R2=0.9956 in the linear range from 277 to 17 pg/μL. The amount of the estrogen receptor alpha (ERα) is determined to be 41 ± 5 pg/μL(Mean ± SD) in the MCF7 cell lysate. However, it is too low to be determined in the A549 cell lysate. The ERα recoveries are calculated to be 102 ± 4 and 94 ± 5 %(Mean ± SD) in the MCF7 and A549 cell lysates from the detection of the spiked sample.
Long-term stability of this plate is determined to be five days by the reproducibility of the calibration curve. In comparison with the commercial product, this plate has longer storage time after the opening.
Furthermore, the miniaturization of this plate can identify β-actin and ERα in the MCF7 cell lysate by the sandwich method via the charge coupled device. Its advantages include the low sample consumption and the real-time detection.

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