本研究主要探討當藍綠菌細胞受到氯破壞後，細胞破損的程度與其胞內代謝物釋出之相關性，實驗以氯做為氧化劑，並以自來水水源中三種常見產臭或毒之藍綠菌做為研究對象，包括微囊藻(Microcystis aeruginosa)、魚腥藻(Anabaena circinalis)，及柱孢藻(Cylindrospermopsis raciborski)，所使用之微囊藻具有產高濃度β-cyclocitral的能力，魚腥藻則會產生geosmin，而柱孢藻所會產生之代謝物目前還未對其進行偵測。實驗前將此三種藍綠菌培養於經數道過濾之金門太湖原水與ASM培養基混合之合成水。
研究中藍綠菌細胞被氯所破壞的程度，以細胞完整性來做表示，並應用螢光染劑FDA(fluorescein diacetate)搭配兩種偵測儀器-流式細胞儀(flow cytometer, FCM)及螢光顯微鏡(epifluorescence microscope, EFM)，測試其完整程度，並應用掃描式電子顯微鏡(scanning electron microscope, SEM)對細胞進行高放大倍率的觀察，以協助判定細胞表面完整情形。在代謝物分析部分，研究中以β-cyclocitral、geosmin分別做為微囊藻與魚腥藻代謝物的代表，並應用固相微萃取法(solid phase micro-extraction, SPME)搭配氣相層析質譜儀(gas chromatograph/mass spectrometry detector, GC/MSD)對此兩種臭味物質進行分析。
研究結果發現氯對於微囊藻及魚腥藻細胞具有較強的破壞效果，其中魚腥藻的抗氯氧化力低於微囊藻，而柱孢藻對於氯則有較強的抗氧化力；整體來說，以氯劑量2 mg/L及4 mg/L對微囊藻及魚腥藻進行氧化，接觸時間30分鐘後會有80%以上的細胞被氯所破壞，但在相同氯劑量下，柱孢藻經過氧化30分鐘後仍有75%以上細胞為完整的。在微囊藻代謝物β-cyclocitral偵測上，發現並沒有隨著細胞被破壞而釋出於水體，原因乃為催化其產生的β胡蘿蔔素加氧脢(β-carotene oxygenase)並氯所抑制的關係，然而geosmin卻會因為魚腥藻細胞被破壞的關係而大量釋出，研究發現原本存在胞內的geosmin約有70-99%，但在氯氧化進行1分鐘後即有超過50%的geosmin釋放於水體中。
以SEM對經氯氧化後的三種藍綠菌進行觀察發現，細胞經氯破壞後會有明顯變形的現象產生，魚腥藻及柱孢藻也會有細胞斷裂的情形發生。

The effect of chlorination on the cell integrity and metabolite release from three nauseous cyanobacteria, including Microcystis aeruginosa, Anabaena circinalis, and Cylindrospermopsis raciborskii, is investigated. The cyanobacteria were grown in the filtrated water from Tai-Lake, Kinmen, with addition of ASM algae growth medium. A fluorescence technique, combining fluorescein diacetate (FDA) with either flow cytometer or epifluorescence microscope, was successfully developed for the determination of cell integrity. A solid-phase microextraction (SPME) concentration followed by a gas chromatograph (GC) and mass spectrometric detector (MSD) was employed to measure the metabolites from the cyanobacteria. A series of chlorination of cyanobacteria-laden water was conducted at different cell concentrations and different chlorine dosages. During the experiments, chlorine concentration, cell integrity, metabolite concentration, and other water quality parameters were monitored at different time. The experimental results revealed that both Microcystis and Anabaena cells are very fragile to chlorine. At an initial chlorine concentration of 2-4 mg/L, almost 80 - >90% of Microcystis and Anabaena cells were ruptured at 2-5 minutes, causing immediate release of metabolites from the cells. For Cylindrospermopsis, however, is more resistant to chlorine. Only ~20% of cells were ruptured at chlorine = 4 mg/L and contact time = 30 minutes. Scanning electro-microscope photos also confirmed that chlorine did have strong impact on the three tested cells. After chlorination, Microcystis cell surface was deformed and became less smooth, and the filamentary Anabaena and Cynlindrospermopsis cells were ruptured and broken into single cells.
The major nauseous metabolite of Anabaena, geosmin, was released into water immediately after the cells were rupture. The geosmin concentrations in the water followed a similar trend of the ratio of ruptured cells, suggesting that geosmin may also serve as an indicator of cell integrity for Anabaena. For Microcystis, the aqueous phase of β-cyclocitral, the major metabolite monitored, did not increased as the cells disrupted for most of the cases, even that most cells were not integral. β-cyclocitral is known to be produced from a rapid reaction of oxygen, β-carotene, and enzymes present in the cells, oxygenase. The chemical is only largely formed after the cells are ruptured. Therefore, it is very likely that the enzymes reacted with chlorine righter after the cells were broken, causing inhibition of β-cyclocitral formation.

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王奕軒(2006)自來水中木頭味物質β-cyclocitral之來源及去除之研究，國立成功大學環境工程學系碩士論文