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研究生: 邱怡如
Chiou, Yi-Ru
論文名稱: 魚鱗膠原蛋白、核醣與殼聚醣以不同比例及交聯順序製備薄膜之性質研究
Characterization of Film Property Prepared from Fish Scale Collagen/Ribose/Chitosan in Different Weight Ratio and Cross-linking Sequence
指導教授: 黃福永
Huang, Fu-Yung
學位類別: 碩士
Master
系所名稱: 理學院 - 化學系
Department of Chemistry
論文出版年: 2021
畢業學年度: 109
語文別: 中文
論文頁數: 75
中文關鍵詞: 膠原蛋白魚鱗交聯核醣殼聚醣
外文關鍵詞: collagen, Fish scales, Cross-linking, Ribose, Chitosan
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  • 摘要Ⅰ 誌謝Ⅵ 目錄ⅤⅡ 表目錄Ⅸ 圖目錄Ⅹ 第一章 緒論 1 1.1 膠原蛋白 1 1.2 魚類膠原蛋白 4 1.3 膠原蛋白傷口敷料簡介 6 1.4 魚鱗膠原蛋白的選用與萃取 7 1.5 膠原蛋白的交聯反應 8 1.6 核醣與殼聚醣簡介 14 1.7 研究動機 16 第二章 實驗 17 2.1 實驗藥品 17 2.2 實驗儀器與設備 17 2.3 從魚鱗處理到製作膠原蛋白膜 18 2.3.1 魚鱗清洗 18 2.3.2 萃取魚鱗膠原蛋白 19 2.3.3 魚鱗膠原蛋白除水乾燥 20 2.3.4 交聯劑配製 20 2.3.5 魚鱗膠原蛋白膜製作 21 2.4 SDS-Page 蛋白質分子量分析 21 2.5 HPLC分析條件 22 2.6 布拉德福蛋白質定量法(Bradford protein assay) 23 2.7 DSC分析方法 23 2.8 膨潤度測試方法 24 2.9 FT-IR分析方法 25 2.10 SEM分析條件 25 第三章 結果與討論 26 3.1 分析兩種魚鱗膠原蛋白之性質差異 26 3.1.1 以SDS-Page 分析魚鱗膠原蛋白 26 3.1.2 以HPLC分析蛋白質分子量 27 3.1.3 蛋白質濃度測定 30 3.1.4 以DSC分析膠原蛋白熱穩定性 31 3.1.5 以FT-IR光譜分析膠原蛋白特徵峰 32 3.1.6 製作膠原蛋白薄膜之比較 33 3.1.7 討論魚鱗膠原蛋白與交聯反應 38 3.2 探討交聯順序對於產物性質的影響 39 3.2.1 以電泳分析交聯順序 41 3.2.2 以SEM分析不同交聯順序之樣品表面 42 3.2.3 以膨潤度分析交聯程度 44 3.2.4 以熱變性溫度分析交聯程度 44 3.2.5 以FT-IR光譜分析交聯反應 46 3.2.6 固定最佳交聯順序找尋最佳反應時間 47 3.2.7 以最佳交聯順序比較兩種魚鱗膠原蛋白薄膜 51 3.3 探討最佳反應比例 53 3.3.1 台灣鯛魚鱗膠原蛋白劑量 53 3.3.2 殼聚醣劑量 56 3.3.3 不同分子量的殼聚醣 59 3.3.4 分子量較小的殼聚醣變化反應時長 63 3.3.5 核醣劑量 66 3.4 與過往研究相比較 68 第四章 結論 70 第五章 參考文獻 71

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