鈦金屬因為具有良好的機械性質與生物親和性，被廣泛的運用在骨科與牙科植體中。但很少的研究指出，不同的純鈦是否具有生物親和性差異。因此本論文有兩大研究目標。第一，利用細胞刮取儀去量化並評估骨母細胞在生醫用純鈦二號、四號的初期黏附力。結果顯示，細胞在純鈦二號上的黏附表現較佳，具有較好的表現。

第二，我們利用不同親水性表面改質技術探討並比較生物親和性差異。從以下方法分析表面性質：利用掃描探針顯微鏡量測粗糙度、以化學分析電子儀分析氧光譜、用靜態接觸角評估親疏水性大小並觀察細胞增殖表現、細胞形態，評估生物親和性。利用低/可變式真空顯微鏡記錄細胞形態，Alamar Blue 檢試劑則是用來測量細胞增殖的表現。

從電子顯微鏡下觀察骨母細胞的細胞形態，經氧氣電漿改質的鈦金屬，細胞有較多的片狀偽足與絲狀偽足生長，可以讓細胞更黏附在材料表面。我們猜測利用氧氣電漿改質鈦金屬表面，將有助於增進細胞黏附力，並有機會提升骨整合速率。

Titanium are widely used in dental and orthopedic implants due to their good mechanical properties and biocompatibility. Few studies indicated that the comparison of biocompatibility between commercially pure (cp) titanium. Thus, we have two goals in this study. First, to evaluate the initial adhesion force of osteoblast between two medical titanium grade 2 and 4 by a cytodetacher. And then, we got the result that the cells seem to prefer the grade 2 titanium and showed the better adhesion force with it.

Secondly, we also compared the effect of biocompatibility on osteoblast by different hydrophilic modification. The surface characterization was measured by below analysis. The surface roughness is measured by scanning
probe microscope (SPM), the chemical properties of surface oxide by Electron Spectroscopy for Chemical Analysis (ESCA), the wettability by static contact angle assessment method. And the cytocompatibility is assessed using cell proliferation and morphology. Scanning Electron Microscopy (LV-SEM) is employed to take images of cell morphology, and cell proliferation is measured by Alamar-Blue assay.

In the SEM images, we have observed that the osteoblast seems to prefer the oxygen plasma-modified titanium and showed more lamellipodia and filopodia grown which could make the cell adhesive. So, we hypothesize that the plasma modification would enhance the cell adhesion and make possible to increase the osseointegration rate.

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