過去研究發現第二型干擾素 （IFN-γ）在硬骨魚類中發現了第二個第二型干擾素而在此簡稱IFN-γrel 。現在因IFN-γ與IFN-γrel的功能差異與性能並不瞭解，因此本研究將探討IFN-γ與IFN-γrel在抗病毒和細菌中扮演的角色。本實驗將選殖出點帶石斑魚osgIFN-γ和osgIFN-γrel (orange-spotted grouper IFN-γ and IFN-γrel) 之序列並有被感染神經壞死病毒的點帶石斑魚作爲試驗材料來探討osgIFN-γ與osgIFN-γrel 在硬骨魚類的抗病毒免疫系統的重要性與兩者之間的差異。利用即時聚合酶鏈式反應測試（Real-Time PCR) ，在受到poly I:C刺激後，osgIFN-γ 基因表現有上升的現象，而在osgIFN-γrel在受到LPS刺激後，基因表現有上升的現象。此外，osgIFN-γ在NNV病毒的攻擊下有強烈的反應，而osgIFN-γrel卻無顯著差異。本研究中也應用突變Mx 的啟動因子裡的GAS 序列來探討IFN-γ與IFN-γrel 對GAS的依賴性，結果顯示只要GAS被突變後IFN-γ與IFN-γrel 對活化Mx的效率明顯下滑。因此本實驗證明了IFN-γ在抗病毒中扮演更重要的角色而IFN-γrel在抗細菌中扮演更重要的角色以及GAS對IFN-γ與IFN-γrel活化Mx的三個啟動因子的重要性。研究結果將對於往後點帶石斑魚在野田病毒的防禦上具有參考價值。

The pass researches of the teleost fish type-II interferons (IFN-γ) founds a second family members of type-II interferons and named IFN-γrel. While the antiviral response of IFN-γ are well characterized, the antiviral response of IFN-γ and IFN-γrel in teleost fish are still not yet fully characterized. Therefore, this study aims to cloned orange-spotted grouper IFN-γ (osgIFN-γ) and IFN-γrel (osgIFN-γrel) sequences and uses NNV infected specimens to study the functionality differences between both IFN-γ with IFN-γrel. By using real-time PCR methods, under the poly I:C stimulations, osgIFN-γ was highly stimulated meanwhile under LPS stimulations the osgIFN-γrel was significantly stimulated. Aside the immunostimulants, under the NNV infection, the osgIFN-γ was significantly more upregulated in contrast of osgIFN-γrel. This study also mutated the GAS sequence found in Mx promoters to study the dependences of osgIFN-γ and osg-IFN-γrel toward it. The results shows that as long as the GAS sequence is mutated, the stimulation of Mx by osgIFN-γ and osgIFN-γrel are significantly less responsive. The results of this study have find that osgIFN-γ and osgIFN-γrel played a different role in pathogen invasion with osgIFN-γ played a more crucial role in anti-viral response, while osgIFN-γrel played a more crucial role in anti-bacterial response. Aside this, this study also found that the important of GAS sequences in activation of Mxs proteins by IFN-γ and IFN-γrel. All of these results would contribute to the prevention of nodavirus infection in orange-spotted grouper for future days.

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