微藻可經由光合作用固定大氣中的二氧化碳並轉化生成生物質，且微藻生長快速，用於環境固碳同時作為生質能源的生產者，是相當具有發展潛力。現今大規模培養藻類主要是以開放式系統為主，其中平面培養池最為普遍。但其二氧化碳利用效能較低；且當微藻濃度變高時，其遮蔽效應變大，造成微藻無法有效率利用光能，使得其培養效率較低。因此，本研究為提高二氧化碳利用效能，使用複合型光生物反應器以增加碳源質傳效率，並於開放式培養槽中放置透明中空矩形槽，以增加培養系統中的光照面積，改變培養系統內的光分佈情況。藉由上述之光生物反應器設計以提高微藻的二氧化碳利用效率與光使用效率，進而提升微藻產率。
本研究結果顯示，於八天連續光照批次培養後，複合型培養系統能有效的提高開放式平面培養池的碳使用效率；插置透明矩形槽可大幅增加微藻的生長速率，使微藻培養能較快到達生長停滯期，並獲得較高的總藻體濃度為0.991 g L-1，此藻體濃度高於一般開放式培養槽約41%。因此，本光生物反應器之設計能有效的改善微藻培養的環境，並提高微藻的產能。另外，研究中為改善藻體沉澱情形，於培養槽中增設間歇性曝氣系統，此系統可透過在槽內提供短暫曝氣的方式，以達到混合的作用。於12小時：12小時(光:暗)光照週期的條件下，比較不同的曝氣操作策略，其結果顯示只於光照期間曝氣之系統，在八天批次培養後，能獲得更高的總藻體濃度與藻體產率(0.816 g L-1與0.089 g L-1 d-1)，且相較於未曝氣之藻體產率可增加約46%。此結果顯示於系統內提供短暫的混合作用能有效的增加微藻產量。關於小球藻培養策略上之結果，重複批次培養系統中，在12小時：12小時光照週期與初始尿素濃度為0.05 g L-1的環境下培養八天，以15%置換比例(補充至0.015 g L-1尿素濃度)培養小球藻，可獲得最高的藻體產率為0.086 g L-1 d-1，與批次培養方式相比其產率增加約31%。

Microalgae have received much attention as renewable energy resources, since the photoautotrophic mechanism can convert the atmospheric carbon dioxide into biomass. Open pond is a general photobioreactor to cultivate microalgae. Nevertheless, the drawback of this system is low carbon dioxide utilization efficiency (CUE), and the increase of microalgae concentration decreases the light penetration in the cultural system. These two reasons result low culture efficiency in open pond system. In this study, the new design of photobioreactor is an open tank containing transparent rectangular chambers (TRCs) combined with bubble column. The hybrid type photobioreactor was developed to improve the photosynthetic efficiency of microalgae and carbon dioxide mass transfer efficiency in the cultural system.
In this study, it was prove that the photobioreactor improved utilization efficiencies of carbon dioxide and light energy for microalgae growth. The biomass concentration of Chlorella sp. reached 0.991 g L-1 on the 8th day. The total biomass concentration obtained was 41% more than open tank system. Besides, the purpose of the intermittence aeration was set up inside the tank in order to enhance the mixing effect in the cultural system. The system with intermittence aeration under the light period resulted in high biomass concentration and productivity (0.816 g L-1; 0.089 g L-1 d-1), and the total biomass productivity was 46% more than that without intermittence aeration system. This result proved that mixing effect can increase microalgae growth in the cultural system. In repeated batch cultivation, the highest biomass productivity was carried out by harvesting the culture with 15% renewal rate and renewing urea at 0.015 g L-1 each time when the cultivation achieved the early stationary phase. After eight day cultivation, the biomass productivity of 0.086 g L-1 d-1 in the repeated batch culture with 15% renewal rate was the highest in comparison with those in the batch and repeated batch cultivations.

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