本研究探討台灣鯛(Taiwan Tilapia)魚鱗粉末之生醫敷膜製備方式及敷膜性質之分析。主要研究利用魚鱗粉末與幾丁聚醣均勻粉混合後，再分別與核醣或梔子素交聯成膜。 由掃描式電子顯微鏡(SEM)微觀其交聯結構；由交聯程度、膨脹率與熱變性溫度(Td)測量其物理性質；以人類腎小管上皮細胞做為細胞增生活性測試。研究結果顯示，最佳化製備魚鱗粉末的條件為：取魚鱗30g以0.01 N 1000 ml的HCl溶液均勻粉碎。實驗時每次取10毫升魚鱗粉粹均質液，再加入不同重量之交聯劑來製膜。實驗發現魚鱗粉末與不同比例的幾丁聚醣的混合膜，再加入核醣或梔子素交聯後，形成的膜擁有較佳的物理特性與細胞增生活性。熱變性溫度試驗，發現在pH11之條件下，以3.15%幾丁聚醣(chitosan)/ 2.36%核醣(ribose)和以3.22%幾丁聚醣(chitosan)/ 0.16%梔子素(genipin)的比例與魚鱗粉末進行交聯後形成的膜有最高的熱變性溫度，分別為97.62 and 98.20 ℃。由SEM觀察發現在pH 7條件下，以8.89%幾丁聚醣(chitosan)/2.22%核醣(ribose)及以0.99%幾丁聚醣(chitosan)/ 0.17%梔子素(genipin)進行交聯所形成的膜具有最好交聯結構，且有大片薄膜狀及較小之孔洞，前者之孔洞平均約18μm，後者約15μm。細胞增生實驗發現在pH9條件下，以0.99%幾丁聚醣(chitosan)/ 0.17%梔子素(genipin) 及在pH8條下，以8.89%幾丁聚醣(chitosan)/2.22%核醣(ribose)比例與魚鱗粉末進行交聯所形成的膜，前者細胞生長速度是未交聯前魚鱗粉末膜的1.89 倍，後者則為4.27倍。因此，使用魚鱗粉末取代由魚鱗萃取出之膠原蛋白來做為生醫敷膜是可行的。

This study is to explore the feasibility of preparing the biomaterial film by using Taiwan Tilapia fish scale powder to cross-link with chitosan and ribose or genipin. The physical properties measured by scanning electron microscopy (SEM), degree of swelling (Swelling ratio), thermal denaturation temperature (Td) were measured. The human kidney proximal tubular epithelial cell line (HK-2) was cultivated on the film to assay the cell growth activity. The optimum condition for prepare the fish scale powder was to grind homogeneously 30g of fish scales in 1000 ml of 0.01N HCl aqueous solution. After mixing homogeneous with various ratios of chitosan, then ribose or genipin in various ratio and different pH value was added to the homogeneous mixture for cross-linkage, followed by lyophilization to make the films. It was found that the film prepared by mixing fish scale powder and chitosan, then cross-linked with genipin or ribose showed better physical properties and cell growth activity. When 3.15% chitosan/ 2.36% ribose and 3.22% chitosan/ 0.16% genipin cross-linked with scale powder under pH 11 condition showed the highest thermal denaturation temperature with 97.62 oC and 98.20 oC, respectively. It was found by using SEM that under pH 7 condition the film prepared by cross-linking with 8.89% chitosan/2.22% ribose and with 0.99% chitosan/ 0.17%genipin showed a typical membrane-like structure and with pore size of 18 μm for the former one and 15 μm for the latter one. The HK-2 cell growth assays found that the films prepared by cross-linking with 0.99% chitosan/0.17% genipin in pH 9 was 1.89 folds higher than that of only fish scale powder film; while cross-linking with 8.89% chitosan/2.22% ribose in pH 8, it was 4.27 folds higher. In this study, we have shown that without extracting the collagen from the scale, by just using the fish scale powder is feasible to prepare the biomaterial film.

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