細胞凋亡在胚胎成長及生理扮演著相當重要的角色，細胞凋亡能夠清除不必要或不正常且無法恢復的細胞，細胞凋亡後會產生凋亡小體由磷脂醯絲胺酸受體(PSR)清除。PSR是一種位於巨噬細胞上用於辨認凋亡細胞來進行吞噬的受體，細胞進行細胞凋亡時位於膜內側的磷脂醯絲胺酸(PS)會翻轉到膜外。
先前研究發現PSR缺陷的斑馬魚胚胎早期發育出現凋亡細胞堆積在體節的情形，並在24小時腦部、眼睛、心臟及體節發育不正常的情形，且利用NGS確定PSR能夠藉由調控凋亡小體來克服氧化壓力進而能讓細胞自噬系統正常運作使中腦以及後腦能正常發育，也確認其與神經可能有進一步的關係。本實驗利用CRISPR/Cas9系統建立PSR剔除魚隻，分析次世代定序(Next Generation Sequencing, NGS)結果發現在神經發育的相關基因都有明顯的改變，並且透過Tg(HuC: EGFP) 轉殖基因魚隻觀察其神經相關基因的表現，在72小時發現斑馬魚胚胎其腦部神經元細胞有訊號減弱的情形產生，同時觀察前中後腦的長度以及腦部面積都有減少的情形，進而利用RT-qPCR觀察其神經發育相關基因表現量與次世代定序結果一致，最後進一步觀察5天大斑馬魚幼魚泳動能力，由結果得知不論是泳動距離、泳動速度以及最大加速度都有受到影響，皆在PSR剔除後下降。綜合結果表示磷脂醯絲胺酸受體缺失會造成神經發育以及運動能力的影響。

Zebrafish is a model system to research the developmental biology of the vertebrate. Researchers have shown that zebrafish and humans have very similar pathological characteristics, so they can be used as animal models for human disease.
Apoptosis is a planned death, which plays an important role in embryo growth and physiological maintenance. Apoptosis can eliminate unnecessary or abnormal and unrecoverable cells. Apoptotic bodies are cleared by the phosphatidylserine receptor (PSR) after apoptosis. Phospholipid serine receptor is a receptor located on macrophages that is used to identify apoptotic cells for phagocytosis. Phospholipid serine (PS) located on the inner side of the membrane will flip out of the membrane when the cells undergo apoptosis. Previous studies have shown that in mouse embryos deficient in phospholipid serine receptors, abnormal development of the brain, eyes, and heart was found.
Previous research had found that the early development of phospholipid serine receptor-deficient zebrafish embryos showed that apoptotic cells accumulated in the somite. And it was found that the phospholipid serine receptor knockout zebrafish at 8 hpf Embryo development is retarded and the concentration of oxygen-active molecules increases, and the brain, eyes, heart, and somite develop abnormally within 24 hpf. And NGS is used to determine that phospholipid serine receptors can be overcome by regulating apoptotic bodies Oxidative stress in turn allows the normal operation of the autophagy system and the normal development of the midbrain and hindbrain, which also confirms that it may have a further relationship with nerves.
Therefore, this experiment establishes the phosphatidylserine receptor gene knock out zebrafish by CRISPR/Cas9 system, analyzes the results of Next Generation Sequencing (NGS), and observes only the expression of nervous system genes through Tg (HuC: EGFP) transgenic fish. As result, some of the nervous genes illustrate downregulation after PSR gene knockout. The transgenic fish Tg (HuC: EGFP) showed that the forebrain, midbrain, and hindbrain area and the fluorescent intensity all reduce after PSR gene knockout. In summary, PSR plays an important role in the early development of the zebrafish nervous system.

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