聚癸二酸丙三醇酯(PGS)彈性體已成功地應用在軟組織工程領域中，是一個同時 具有彈性、生物相容性、生物降解性與血液相容性的材料，然而要製備出纖維型態的 PGS是相當困難的，PGS彈性體無法溶解於溶劑中，PGS預聚物因分子量低，溶液黏 度低，無法單獨被電紡。我們以同軸靜電紡絲法製備出具纖維結構的PGS薄膜，使用 可以被電紡的高分子溶液(PEO/PLA)作為殼層，帶出不可被電紡的芯層(PGS預聚物)， 調整內芯70wt% PGS預聚物的溶液黏度和提升外殼溶液中PLA的含量，以穿透式電子 顯微鏡與掃描式電子顯微鏡分別確認了芯殼的纖維結構與纖維型態，改善了同軸靜電 紡絲的條件，降低PGS預聚物在高溫交聯時外漏的可能。透過紅外光譜分析儀與示差 熱掃描分析儀確認PEO與PLA經過一系列處理步驟後皆完全被移除。我們製備出具高 度順向性的PGS纖維薄膜，以單軸拉伸測試與纖維方向平行拉伸及與纖維方向垂直拉 伸的力學性質，結果顯示出非等向性(anisotropic)的力學性質，其楊氏模數(0.52-0.98MPa)與軟組織相近。電紡纖維對人類臍動脈平滑肌肉細胞產生了細胞接觸 引導的能力，能夠模仿天然組織結構。我們亦透過Alamar blue與LDH assay證實其具 有良好的生物相容性，可於組織工程的領域中有著多元的應用。

Although we have successfully fabricated poly(glycerol sebacate) (PGS) fibrous membrane by coaxial electrospinning, it remains PGS prepolymer leak-out issue during the fabrication process. In this study, we tended to reduce the ratio of PEO to PLA in the shell solution or use more intensively crosslinked pPGS. On the other hand, one of the advantages of using fibrous scaffolds in tissue engineering is that cell alignment and hence tissue microstructure may be manipulated by the mechanism of contact guidance. Therefore, we focused on fabricating highly aligned PGS fibrous membrane. The core-shell fiber structure was confirmed by TEM. From the SEM images, we successfully mitigated pPGS leak-out from the inner core. Both of PEO and PLA were removed from the PGS fibrous membrane, which were verified by FTIR and DSC. The alignment index of PGS fibrous membrane was 2.06±0.21 indicating that PGS fibers were highly aligned. From the results of cyclic stretching test, we further affirmed that PLA was removed. PGS fibrous membrane also showed anisotropic mechanical properties examining by uniaxial tensile test. It’s Young’s modulus was 0.52-0.98MPa which was similar to soft tissues. Anisotropic PGS electrospun fibers promoted human umbilical smooth muscle cell guidance. We discovered that the degradation rate of PGS fibrous membrane was slower than PGS solid sheet, indicating that the crosslinking degree of PGS fibrous membrane might be higher than PGS solid sheet. The in vitro cytocompatibility of the electrospun membranes was confirmed. The aligned PGS fibrous membrane has the potential to be used for soft tissue engineering applications

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