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研究生： 鄭耿閔
Cheng, Keng-MIng
論文名稱： 雙面不對稱球體之自組裝與其螢光共振能量轉移效應之研究
Self-assembly and FRET of Dye-functionalized Janus Particles
指導教授： 郭昌恕
Kuo, Changshu
學位類別： 碩士
Master
系所名稱： 工學院 - 材料科學及工程學系
Department of Materials Science and Engineering
論文出版年： 2013
畢業學年度： 101
語文別： 英文
論文頁數： 80
中文關鍵詞： 雙面不對稱球體 、自主裝 、螢光共振能量轉移 、光致發光
外文關鍵詞： Janus particle, Self-assembly, Fluorescence Resonance Energy Transfer, Photoluminescence
相關次數： 點閱：58  下載：0 
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    • 本篇論文探討雙面不對稱球體的自發性自主裝及螢光共振能量轉移效應。雙面不對稱球體是以粒徑為500奈米之二氧化矽球體將其半球表面進行氨基改質，接著再接上稱為Marina blue的螢光染料，並與購買的100奈米之表面羧酸化聚丙烯腈微珠作自發性自組裝。這些聚丙烯腈奈米微珠已經預先帶有Chromeon 470之螢光染料並可以當作螢光共振能量轉移的受體物質，並同時以Marina blue作為螢光共振能量轉移的供體物質。球體在水溶液中的自主裝行為是由500奈米之帶有正電荷的不對稱球體與100奈米之帶有負電荷的聚丙烯腈微珠進行自組裝，自組裝後之粒徑大小可以利用動態光散射粒徑分析儀觀測，同時藉由自組裝後使供體與受體物質之間的距離縮短而引起螢光共振能量傳遞行為，其中距離是螢光共振能量傳遞最重要的參數。藉由螢光光譜測量激發與吸收光譜來驗證在球體的自組裝下能夠順利引起螢光共振能量傳遞。

    In this research work, asymmetric Janus particles were fabricated from the investigation of the self-assembly and the fluorescence resonance energy transfer (FRET). Janus particles cored with 500 nm silica colloids were functionalized with amino groups on one of their hemispheres, followed by the attachment with fluorescence dye, Marina Blue. These Janus particles in the submicron scale were carefully fabricated and characterized. Commercially-available polyacytlonitrile (PAN) particles with 100 nm in diameter and carboxylic surface-functionalities were employed as the self-assembly counterpart. These PAN particles were pre-loaded with another fluorescence dye, Chromeon 470, served as the FRET acceptor, while the Marina Blue Janus particles were utilized as the FRET donor. Self-assembly of these particles in an aqueous solution were initiated by the positively-charged asymmetric 500 nm Janus particles and the negatively-charged 100 nm PAN particles. The assembly and the cluster sizes were monitored by the dynamic light scattering. Meanwhile, the formation of the assembled clusters shortened the distance between the donor and acceptor, that was in fact the most crucial parameter for the FRET. Photoluminescence for both excitation and emission spectra indicated the successful FRET as these two particles process the self-assembly.

    致謝 I 中文摘要 II Abstract III Table of Contents IV List of Illustrations VII Chapter 1. Introduction 1 1.1. Janus particles and asymmetric micro- and nano- materials 1 1.1.1. Janus-like Materials 2 1.1.2. Fabrications of Janus Particles 6 1.1.3. One-dimensional Electrospun Fibers as Particle Embedding Substrates 15 1.1.4. Applications 18 1.2. Self-Assembly 22 1.2.1. Introduce to Self-assembly 22 1.2.2. Models of Self-Assembly 25 1.2.3. Self-assembly Simulations 30 1.2.4. Applications of Self-Assembly 31 1.3. Förster Resonance Energy Transfer 35 1.3.1. Mechanism and Applications 36 1.3.2. Assembly of Dye-functionalized Janus Particles 40 1.4. Research Motivations 41 Chapter 2. Experimental 43 2.1. Chemicals 43 2.2. Instruments Used in the Material Fabrication 45 2.3. Preparations of Dye-functionalized Janus Particles 47 2.3.1. Preparation of 500 nm Silica Particle Solution 47 2.3.2. Electrospinning of PMMA/P4VP Blend Fibers 47 2.3.3. Dipping Process of 500 nm Silica Particles 48 2.3.4. Standard Film Preparation 48 2.3.5. Heat Treatment Process (Thermal Embedding Process) 49 2.3.6. APS Modification Process 49 2.3.7. Polymer Fibers Dissolving Process 50 2.3.8. Dye Grafting Process 51 2.4. Analytical Instruments 52 2.4.1. UV-vis Spectrometer (UV-vis) 52 2.4.2. Photoluminescence Spectroscopy (PL) 52 2.4.3. Scanning Electron Microscopy (SEM) 54 2.4.4. Particle Size Distribution 54 2.4.5. Zeta Potential Analyzer 55 Chapter 3. Results and Discussions 56 3.1. Dye-functionalized Janus Particle 56 3.1.1. Amino Janus particle 56 3.1.2. Dye-functionalization 60 3.2. Self-assembling Behaviors of Janus Particles and Carboxylic Nanobeads 61 3.3. FRET Effect of Assembled Particles 64 3.3.1. FRET of Marina blue and Chromeon 470 nanobeads 64 3.3.2. FRET of Dye-functionalized JPs and Chromeon 470 nanobeads 68 Chapter 4. Conclusions 73 References 74

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