目前有很多關於表面增顯拉曼散射的研究，其中許多研究所開發的表面增顯拉曼散射基板都有不錯的拉曼增強效果，但要同時達到高再現性與均勻性的增強效果並不容易，而這是表面增顯拉曼散射在實際應用時所面臨之挑戰。本研究將奈米微粒與透過DNA自組裝成規則且緊密排列的三維晶體結構，透過此結構增強拉曼訊號以檢測小分子。由於奈米銀相較於奈米金更可以增強拉曼光譜訊號，我們將奈米銀包覆於奈米金粒周圍，並透過改變銀殼的厚度來調整兩相鄰奈米粒子間之距離，尋找出最合適之間距，藉此達到穩定且良好的拉曼增強效果。

At present, lots of people do the research of the surface-enhanced Raman scattering, and they also got the great Raman enhancement effect. But, it is not easy to synthesize high reproducibility and uniformity structures. If we want to apply the surface-enhanced Raman scattering signal into development, we have to overcome this challenge. This study use nanoparticles and DNA to synthesize the self-assembly of 3-dimensional crystal structure, which are able to order regularly and closely. According to those associated studies, silver and gold nanoparticles are able to enhance abundantly signals in Raman spectra. Compare gold to silver, silver has more enhancive effect than the gold. Hence, we use silver to pack around the gold nanoparticles. We can enhance Raman signal successfully through adding different length or sequences of DNA and adjusting the silver thickness around nanoparticles which will affect the gap between two nanoparticles. We want to find the best stability enhancements, and produce the reproducibility and uniformity structures.

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