本研究提出一個新型的光學二維量測系統; 以史托克向量與穆勒矩陣為理論基礎，利用CCD發展一個二維的量測模型，並透過differential matrix model 來求出線性雙折射、線性雙向衰減、旋性雙折射及旋性雙向衰減及去偏極化等九個光學參數。相較於以往的分析模型，本系統藉由二維的量測系統來對於包含散射介質的異向性光學特性的生物樣本進行分析。此二維的量測模型是針對異向性光學特性的生物細胞進行多參數量測; 而此多參數模型可更精準量測其線性雙折射特性的變化。值得注意的是，以往的線性雙折射分析模型 (光彈模型) 會直接忽略其他非線性雙折射特性所造成的誤差影響。
本研究的測試樣本分為兩個部分。第一部分以二維光學量測模型結合顯微鏡系統，從巨觀以及微觀的方面來量測具有不同抗藥性的卵巢癌與子宮頸癌細胞。量測不同抗藥性之癌細胞是否有不同的光學性質，並利用顯微鏡系統從微觀方面量測單一細胞以了解巨觀方面所得到的差異是否來自於細胞本身。此研究進行了沒有人探討過的不同抗藥性的卵巢癌與子宮頸癌細胞之光學性質，期望可以應用於臨床上，判別癌細胞所具有抗藥性。
第二部份以二維量測模型以及微型拉伸試驗機來進行生物組織的拉伸試驗，觀察在受到拉力的情況下，生物組織的光學參數如何改變。我們以雞胸肉組織作為量測樣本，並建立了一系列的光學模型來探討線性雙向衰減(LD)、旋性雙折射(CB)、旋性雙向衰減(CD)以及去偏極化(Dep)在不同運算模型下是如何影響單以光彈理論所獲取的線性雙折射(LB)之正確值。

This study develops a new type of experimental scheme to extract optical properties by Stoke vector and Mueller matrix on biotissue and cancer cell. Using CCD camera for a two-dimensional measurement and applying differential matrix model to decouple and analyze the optical properties including linear birefringence (LB), linear dichroism (LD), circular birefringence (CB), circular dichroism (CD) and depolarization (Dep) properties of turbid media is introduced. Different from previous optical models, this system is to use two-dimensional full-field ellipsometry to measure anisotropic optical sample. It is noted that previous optical model such as photoelasticity model usually ignores the other properties on anisotropic biotissue.
In this research, we have two parts for research. In the first part, the cervical and ovarian cancer cells with different chemoresistance is observed in macroscopic and microscopic images. Measuring the different optical properties between the ovarian cancer cells with different chemoresistance is to figure out how those differences come from by the macroscopic and microscopic images. This study is focus on the optical properties of cancer cells with different chemoresistance and this part has never been done before, and we hope that this technique can be applied on clinical treatments to distinguish the chemoresistance cancer cells.
In the second part, we combine two-dimensional optical system and a home-made stretching machine for conducting the experiment on the biological muscle. The chicken breast muscle is chosen to figure out how the optical properties change on the stretching biological tissue. After experiments, we build up a series of simulations to observe how linear dichroism (LD), circular birefringence (CB), circular dichroism (CD) and o depolarization (Dep) affect the linear birefringence (LB) if only the photoelasticity model is applied without considering the other existing optical properties.

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