近年來氣候變遷，乾旱頻傳，植物抗逆境的研究重要性日漸重要。除了植物本身的生理調控之外，植物-微生物交互作用的研究也受到注目。根內菌是指一群微生物生長在根內卻不造成疾病。許多研究提及根內菌對於植物的生物量、抗逆境能力有提升的效果。芒草以生長速度快、抗逆境等特性而聞名。本次的研究材料五節芒可以生長在惡地與一般草地，而前人研究提到不同棲地的芒草會有不同的根內菌組成。本研究探討五節芒生長在惡地與一般棲地組成差異，並分離內生菌。首先，在豐富度方面， Actinobacteriota 占惡地環境根內的50%，通常環境根內、惡地環境根圈土、通常環境根圈土都是Proteobacteria 占大多數。放線菌門的增加來自40種Amplicon sequence variants (ASV)，這些ASV可以在根內與根圈土被發現，其中有20%是鏈黴菌屬 (Streptomyces) 。比較這些ASV不同環境的豐富度差異，有超過8成是根內比率大於根圈土比例，顯示在根內具有某種程度的篩選。用 clam test 分類之後，根內specialist放線菌明顯擴增，而根圈土specialist則是Proteobacteria明顯擴增。以功能性分析結果使用PCA分析clam test組別並未發現明顯差異。分離出的內生菌的組成與定序所得到的結果相差甚大，突顯出分離法與定序法的落差。本研究顯示出不同環境的內生菌組成差異。

Droughts have been becoming serious in the past decades. Endophytes are a group of symbiotic microbes in roots. Many research suggest that endophytes increase biomass, abiotic stress tolerance and of plants. Miscanthus is characterized by high growth rates, high abiotic stress tolerance. In the study, floridulus is able to grow in badland and regular habitat and has different endosphere microbiome under different environments. I investage the differences in endosphere microbiome between Miscanthus floridulus population at badlands and regular habitat and isolate drought stress related endophytes for further plant drought stess research. In endosphere from badlands, there are over 50% abundance attributable to Actinobacteriota. Nevertheless, Proteobacteria is most abundance in other sites, endosphere from regular habitat, rhizosphere from badlands and rhizosphere from regular habitat. The increase of Actinobacteriota in endosphere of badlands caused by 40 ASVs and these ASVs can be detected in both endosphere and rhizosphere. Microbiome compostion has no different among four groups. Shannon index, Chao1 richness index and NMDS plot display significant different between endosphere and rhizosphere. PICRUST2 analysis only present results in rhizosphere. After clam test analysis, specialist in endosphere show significnant enrichment in Actinobacteriota while specialist in rhizosphere show significant enrichment in Proteobacteria. The result shows the differences of endosphere microbiome.

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