本研究成功地結合微流體技術及電化學感測方法在一微流體晶片中來偵測尿蛋白。此微流體晶片由自動檢體稀釋晶片模組與尿蛋白檢測晶片模組組成。自動檢體稀釋晶片模組由疏水閥門、氣動式閥門與微流管道所構成，主要負責在實驗時調配所需的檢體濃度；尿蛋白檢測晶片模組則由氣動式環形幫浦、微流管道與尿蛋白感測晶片組成，負責檢體的傳輸與尿蛋白的偵測。
在晶片製作方面，二模組所需的微流體控制元件包含微流管道、疏水閥門、氣動式閥門、氣動式環形幫浦，皆以聚二甲基矽氧烷（Polydimethylsiloxane, PDMS）材料製作，而感測電極係利用電聚合方法在白金電極上電聚合吡咯（pyrrole, PY）和胺苯硼酸（aminophenylboronic acid, APBA）薄膜完成，最後再利用氧電漿將上板微流體晶片與感測電極封裝完成。
　　研究中利用十倍的磷酸鹽緩衝液（Phosphate Buffer Saline, PBS）（pH=5）來模擬人體尿液，並以白蛋白做為感測目標物，運用此微流體感測晶片做快速偵測，並觀察溶液中是否有尿蛋白的存在，以判斷人體的健康情況。研究之最終目標是希望藉由此系統的發展，提供一個快速檢測的平台技術。

This paper presents a new microfluidic system capable of detecting urine proteins in an automatic fashion. The system integrated with two major functional devices including a sample dilution module and a urine protein detection module. The sample dilution module consisting of a hydrophobic valve, microvalves and microchannels is designed for diluting the concentration of protein samples. The urine protein detection module is composed of a new spiral-shape micropump, microchannels and an electrochemical sensor for urine protein detection. This module is used for transporting the protein sample solution and for detecting the urine proteins inside the solution. All of the microfluidic structures in the system are fabricated by using soft lithography of polydimethylsiloxane (PDMS). Then, the electrode for the urine protein sensor is formed by depositing and patterning a thin-film of platinum (Pt), followed by electro-depositing a thin polypyrrole layer and poly-aminophenylboronic acid to form the sensing layer. Finally, the sample dilution module and the urine protein detection module are bonded together using the oxygen plasma treatment to form an integrated system. Besides, the proposed integrated system can successfully detect the human urine proteins with a detection limit of 0.1 ppm, As a whole, this study may provide a powerful platform for electrochemical detection of urine proteins.

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