膠原蛋白為天然的生醫用材料，具有優異的生醫功能特性，可促進軟組織修復或使傷口癒合而無痂疤的形成；其多種應用因此已見於保健與美容相關方面。為降低使用含膠原蛋白衍生品的成本，其它途徑，例如：以水解膠原蛋白片段取代成為類似膠原蛋白物質或將之固定在高分子基材上的方式，已經廣被考慮。水解膠原蛋白較缺乏生物可反應性，但在護膚保濕的能力則廣被認可。固定膠原蛋白或水解膠原蛋白經常藉由共價鍵結的方法，然而，固定膠原蛋白的生物活性會隨著存放時間而退化。除此之外，含膠原蛋白製品仍需經過滅菌處理，此可能使得膠原蛋白結構受到破壞，造成其生物活性再降低至某種程度。本研究利用氧氣電漿活化聚丙烯不織布，隨之，將之接枝丙烯酸單體使修飾的表面具官能基與親水性的結構，再以媒合劑固定Type III膠原蛋白或水解膠原蛋白片段於其上。使用減弱全反射-傅立葉轉換紅外光譜儀及X光光電子能譜儀對改質後之表面進行化學分析，實驗結果顯示：接枝丙烯酸的羧基（O=C-OH）與兩種膠原蛋白分子均可形成共價鍵結的醯胺鍵結（O=C-NH）。由於生物分子大小的不同，水解膠原蛋白之固定量較Type III膠原蛋白多約4.2 μg/cm2；然而，Type III膠原蛋白仍保有促凝血之生物活性，水解膠原蛋白則無。兩種固定膠原蛋白之試片可在照射254 nm的紫外線20小時後達到滅菌的效果；同時，固定的膠原蛋白之大部分的生物活性仍然保有。在含膠原蛋白物質中可以抑制細菌生長的添加物可以有效地降低紫外線照射所需時間，因而更可保有固定膠原蛋白的生物活性。

　　Collagen is a natural biomaterial that retains excellent bio-functional properties in repairing soft tissues or healing wound without scar formation. Many applications have therefore been relevant to health care as well as cosmetics. To reduce the cost of utilizing collagen-containing derivatives, alternative solutions such as replacing dehydrated collagen segments as a collagen-like substance or immobilizing collagen upon a polymeric substrate have been widely considered. The dehydrated collagen segments are relatively short of bio-reactivity, however, its capability of upholding moisture for skin care is generally recognized. To immobilize collagen or dehydrated collagen segments, the covalently bonded method is usually utilized, whereas the bioactivities of collagen are likely degraded along storing time. In addition, collagen-containing substances should be validated to a sterilization process that may provoke structural damages of collagen and thus reduce its bioactivity to a certain degree. In this study, O2 plasma was utilized to activate non-woven polypropylene (PP) fabric and then the fabric was grafted co-polymerized with acrylic acid (AAc) to obtain a modified surface with functional and hydrophilic structure. Collagen type III or dehydrated collagen segments was then immobilized on the pAAc-grafted fabric using coupling agents. Fourier-Transformed Infrared with Attenuated Total Reflection and X-ray Photoelectron Spectroscopy were employed for surface analyses on the modified samples, experimental results demonstrated that the O=C-OH group from pAAc was covalently bonded with both collagens and formed the amide bond (O=C-NH). Owing to the variation of bio-molecular sizes, the immobilized quantity of the dehydrated collagen segments was �l4.2 μg/cm2 more than that of Type III collagen. Using blood-clotting tests, the immobilized Type III collagen still retained their original bioactivities; however, little bioactivity was found for the immobilized dehydrated collagen segments. Both collagen-immobilized fabrics could be sterilized after 20 hrs of UV-254 nm irradiation, while the bioactivity of the immobilized collagen was mostly preserved. A chemical additive that was effective to inhibit bacterial proliferation in the collagen-containing substances could also reduce the required UV exposure time and therefore significantly uphold its bioactivity.

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