磁性奈米材料相關的研究已廣泛地應用於生物醫學上。由於其超順磁特性及低生物毒性，在外加磁場下，可作為體內傳遞藥物的載體，並因其熱效應，能夠有效地抑制或除去癌細胞，更以標靶癌細胞的方式，可使藥物及熱治療直接對位到癌細胞。
本研究是以水相共沉澱法製備磁性奈米粒子，在其外層包覆β-環糊精 (β-cyclodextrin, β-CD) 及亞麻油酸 (linoleic acid)，以探討奈米磁球的熱效應；並以環糊精疏水性的中孔包覆抗癌藥物，而亞麻油酸可標靶肝細胞。以XRD (X-ray diffractometer) 確認奈米磁粒為Fe3O4之晶相，而利用FT-IR (Fourier Transform Infrared Spectrophotometer) 與TGA (Termogravimetric Analysis) 鑑定β-CD及亞麻油酸已修飾於其上，並以TEM (Transmission Electron Microscope) 影像分析估算Fe3O4、Fe3O4@β-CD及Fe3O4@β-CD-LA粒子之平均微晶尺寸，分別為8.87  4.43 nm、9.97  3.86 nm與11.28  1.52 nm。由磁性分析之磁滯曲線可以得知Fe3O4、Fe3O4@β-CD及Fe3O4@β-CD-LA的飽和磁化量分別為84.51 emu/g、68.24 emu/g與27.89 emu/g。繼之將奈米磁球進行滅菌，探討滅菌後對組織細胞的影響。已有文獻指出capsaicin對癌細胞有明顯的抑制效果，特別是前列腺癌細胞，因此也進行修飾後的奈米磁球對包覆capsaicin以及釋放的探討。
奈米裸磁粒在施加高頻磁場下，於10分鐘內可達90oC，在50分鐘內可達100 oC；而經由β-CD包覆後，則10分鐘內可達72oC；而經由β-CD與亞麻油酸修飾後則在10分鐘內可達66oC，顯示經包覆後的奈米磁粒，其熱效應明顯下降，但還是具有良好之效果足以進行對腫瘤細胞之熱治療。而經過滅菌後的奈米磁性粒子於10分鐘高頻磁場下分別可達94 oC (Fe3O4)，68 oC (Fe3O4@β-CD)，以及64 oC (Fe3O4@β-CD-LA)，顯示滅菌後依然具有良好熱治療效果。

The research of magnetic nanoparticles has been extensively applied to biologically medical technology for several years. The nanoparticles can be used as a drug carrier due to their size-dependent superparamagnetism and low cytotoxicity. Additionally, their hyperthermia anticancer treatment can depress or kill the cancer cells. And the use of targeting technique makes the drug and hyperthermia treatment directly affects the cancer cells.
In this study, the magnetic nanoparticles were synthesized by co-precipitation. The surface of nanoparticles was further modified with β-cyclodextrin and linoleic acid. Cyclodextrin, known to be a good host for the inclusion of guest molecule. Linoleic acid can be used to target hepatocytes. The thermal effect of the magnetic nanoparticles under applied magnetic field was also investigated. We used XRD to confirm the Fe3O4 structure of magnetic nanoparticles, and the modification by β-CD and linoleic acid was confirmed by TGA and FT-IR. The size of Fe3O4、Fe3O4@β-CD and Fe3O4@β-CD-LA were estimated to be 8.87  4.43 nm、9.97  3.86 nm and 11.28  1.52 nm, respectively, which were observed and calculated by TEM. SQUID results indicated that saturation magnetization of Fe3O4 、Fe3O4@β-CD and Fe3O4@β-CD-LA were 84.51 emu/g 、68.24 emu/g and 27.89 emu/g, respectively. Then we proceed to sterilization by autoclave and investigate the influence on tissue cells. Some researches have showed that capsaicin can depress the cancer cells, especially prostate cancer cells. So we also investigated the loading and releasing efficiency of capsaicin.
Under applied magnetic field, temperature of bare magnetic nanoparticles could increase up to 90 oC in 10 min and 100 oC in 50 min. With the modification of the nanoparticles with β-CD, the temperature could increase to 72 oC in 10 min. And with the modification of the nanoparticles with β-CD-LA, the temperature could increase to 66 oC in 10 min. The thermal effect of magnetic nanoparticles with modification could remarkably decrease, but they still have excellent hyperthermia effect toward cancer cells.

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