臺灣龍膽石斑魚(鞍帶石斑魚)為一產量高且高經濟價值之養殖魚類，由於近年來養殖技術之成長，擁有穩定之產量，而乳酸菌發酵液具有抗氧化的功能，且被認為是一非常安全的菌種，它的抗氧化能力並非只其菌種本身，而是經過發酵代謝出的物質具有生理機能，固本實驗以龍膽石斑受精卵萃取物當作發酵基質，希望透過乳酸菌發酵產物來提升受精卵萃取物的生化活性，進而發展為高附加價值之抗氧化產品。
首先培養三種不同來源之乳酸菌當作發酵母菌體，對處理後受精魚卵萃取液進行發酵8、12及24小時，以SDS-PAGE蛋白質分析發現在13、26、36及70 KDa左右的蛋白質被水解而濃度變淡剰至消失的現象，在抗抗氧化力方面，經過三種來源之乳酸菌都是隨時間增加而有所提升，其中以AB優酪乳培養之乳酸菌有最好的自由基清除率(IC50為0.3372 mg/ml)跟還原力，而乳酸菌酵素粉培養之乳酸菌效果則是最差，在抗氧化物質含量分析發現，其自由基清除率與還原力都與多酚含量呈正相關性，隨著發酵時間而有增加的趨勢。

Taiwan Epinephelus Lanceolatus is a high economic-valued, high production cultured fish species. In the last decade, the farming technique of Taiwan Epinephelus Lanceolatus has been promoted and shown a steady production. Taiwan Epinephelus Lanceolatus is the biggest, having high amount of proteins of Taiwan cultured fish species. Lactic acid bacteria has been recognized generally as a safe food supplement. According to studies, after lactobacillus fermented, it will metabolism some anti-oxidative bioactive compounds, including peptides, polyphenols and polysaccharides. Therefore, in this study the bioactivity of the zygote extract from Epinephelus Lanceolatus after incubation with Lactobacillus to was assayed and the content of the anti-oxidative bioactive compounds was analyzed.
The SDS-PAGE was used to analyze the protein components of the zygote extract before and after incubated with lactobacillus from three different sources. It was found that the incubated extract shows some protein was hydrolyzed under 13, 26, 36 and 70KDa. The anti-oxidative results showsfound that anti-oxitive and reductive activities increases as the incubation (fermination) time increases. And the zygote extract after fermented with lactobacillus from commercial yoghurt has the highest anti-oxidative and redctive activities. Finally, the content of the anti-oxidative bioactive compounds analysis It was found that anti-oxidative and reductive activities are proportionally related to the contents of the polyphenols.

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